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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
OECD guideline study with some deviations. GLP compliant

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Deviating from the OECD Guideline 209 without 3,5-Dichlorophenol as reference substance and one used potassium dichromate. A concentration was used, which causes 50% inhibition of the Respiration.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
not specified
Water media type:
not specified
Total exposure duration:
3 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
100 mg/L
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
100 mg/L
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
580 mg/L
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L

Any other information on results incl. tables

Remarks: No repetition of the analysis, since those exhibits test substance a weak increase in the process of the concentration ratio effect.

Criteria for the validity of the test:

1. Difference of the control respiration rates:< 15%

2. Inhibitor effect of the reference substance concentration: 40 - 60%

Reference substance: Potassium dichromate p.a. Merck

Test concentration: 60 [mg/L test medium]

Reference inhibition: 51 [%]

Validity criterion: Inhibition between 40 and 60%

Applicant's summary and conclusion

Conclusions:
EC10=100 mg/L, EC20=100 mg/L, EC50= 580 mg/L, EC80 = >1000 mg/L
Executive summary:

An activated sludge respiration inhibition test was conducted in accordance with OECD Guideline 209. The 3-hour EC50 was 580 mg/L.