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EC number: 208-792-1 | CAS number: 541-73-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- The study deviates from the TG by not using one of the following strains E. coli WP2 uvrA, or E. coli WP2 uvrA (pKM101), or S. typhimurium TA102.
Data source
Reference
- Reference Type:
- publication
- Title:
- Structural specificity of aromatic compounds with special reference to mutagenic activity in Samonella thyphimurium- a series of chloro- or fluoro-nitrobenzene derivatives
- Author:
- Shimizu M, Yasui Y, and Matsumoto N
- Year:
- 1 983
- Bibliographic source:
- Mutat. Res. 116, 217-238
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Rats were injected with PCB instead of Aroclor 1254. The S. thyphimurium strains, TA98, TA1538, TA1537, TA100 and TA 1535 were used instead of TA1535, TA1537 (or TA97a or TA97), TA98, and TA100 , and E.coli WP2 strains or S. typhimurium TA102.
- Principles of method if other than guideline:
- The mutagenicity of chlorobenzene compounds in Salmonella typhimurium (strains TA98, TA1538, TA1537, TA100 and TA1535) was examined.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,3-dichlorobenzene
- EC Number:
- 208-792-1
- EC Name:
- 1,3-dichlorobenzene
- Cas Number:
- 541-73-1
- Molecular formula:
- C6H4Cl2
- IUPAC Name:
- 1,3-dichlorobenzene
- Details on test material:
- IUCLID4 Test substance: no data
- Name of test material (as cited in study report): m-dichlorobenzene
- Analytical purity: 98%
Constituent 1
Method
- Target gene:
- his
Species / strain
- Species / strain / cell type:
- other: TA 98, TA 100, TA 1535, TA 1537, TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 0.02, 0.04, 0.08, 0.16, 0.32, 0.64, 1.28, 2.56 µL
- Vehicle / solvent:
- - Vehicle used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: N-Ethyl-N´-nitro-N-nitrosoguanidine (ENNG), 2-nitrofluorene (2-NF), 9-aminoacridine (9-AA) and 2-aminoanthracene (2-AA)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
NUMBER OF REPLICATIONS: all test were performed in duplicate and repeated at least 3 times - Evaluation criteria:
- Colonies of his+ revertants were counted after incubation, and chemicals inducing more than twice the number of revertant colonies on the control plate were considered as mutagenic.
- Statistics:
- Not performed
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Vehicle controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'. Remarks: TA 98, TA 100, TA 1535, TA 1537, TA 1538
Any other information on results incl. tables
Mutagenicity of 1,3-dichlorobenzene in S.typhimurium TA 98, TA 1538, TA 1537, TA100, and TA 1535
Dose per plate µL |
His+ revertants/plate |
|||||||||
|
TA98 |
TA 1538 |
TA 1537 |
TA 100 |
TA 1535 |
|||||
|
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
0.02 |
30 ± 3 |
29 ± 4 |
24 ± 4 |
24 ± 5 |
8 ± 2 |
6 ± 2 |
180 ± 22 |
228 ± 22 |
36 ± 5 |
32 ± 4 |
0.04 |
28 ± 3 |
33 ± 7 |
22 ± 3 |
18 ± 3 |
13 ± 3 |
9 ± 2 |
172 ± 16 |
196 ± 17 |
31 ± 4 |
38 ± 6 |
0.08 |
30 ± 5 |
24 ± 3 |
21 ± 3 |
22 ± 3 |
10 ± 2 |
9 ± 2 |
135 ± 25 |
190 ± 15 |
32 ± 5 |
30 ± 3 |
0.16 |
21 ± 3 |
29 ± 5 |
17 ± 2 |
30 ± 6 |
8 ± 2 |
8 ± 2 |
142 ±18 |
186 ±18 |
29 ± 4 |
24 ± 3 |
0.32 |
23 ± 4 |
22 ± 3 |
25 ± 5 |
20 ± 2 |
9 ± 2 |
11 ± 4 |
154 ± 13 |
177 ±16 |
22 ± 3 |
28 ±4 |
0.64 |
17 ± 2 |
25 ± 4 |
21 ± 4 |
31 ± 5 |
10 ± 3 |
9 ± 2 |
156 ±18 |
181 ± 17 |
28 ± 5 |
24 ± 3 |
1.28 |
15 ± 3 * |
20 ± 3 |
0* |
2± 8* |
3 ± 3* |
5 ± 3* |
155 ± 9 |
164 ±14 |
6 ± 8* |
17 ± 3 |
2.56 |
0* |
0* |
0* |
0* |
0* |
0* |
0* |
42 ±22* |
0* |
0* |
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of the study, the test item was not mutagenic and not classified according to CLP Regulation (EC) 1272/2008.
- Executive summary:
The mutagenicity of 1,3-dichlorobenzene was examined in Salmonella typhimurium (strains TA98, TA, 1538, TA 1537, TA 100, and TA 1535). The test was carried out with and without liver microsomal activation.
1,3-dichlorobenzene showed no mutagenic activity and toxicity at higher doses.
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