1,3-dichlorobenzene

Long-term toxicity to fish

Administrative data

Endpoint:

long-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Toxicity of Chlorinated Benzenes to Fathead Minnows (Pimephales promelas)

Author:

Carlson AR & Kosian PA (1987): Arch. Environ. Contam. Toxicol. 16, 129-135

Year:

1987

Bibliographic source:

Arch. Environ. Contam. Toxicol. 16, 129-135

Reference 2

Reference Type:

grey literature

Title:

Unnamed

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: APHA (1971): Standard Methods for the examination of water and wastewater

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

5431-73-1

IUPAC Name:

5431-73-1

Constituent 2

Reference substance name:

1,3-dichlorobenzene

EC Number:

208-792-1

EC Name:

1,3-dichlorobenzene

Cas Number:

541-73-1

Molecular formula:

C6H4Cl2

IUPAC Name:

1,3-dichlorobenzene

Test material form:

other: liquid

Details on test material:

- Purity: 98 % (Aldrich Chemical Company, Milwaukee,USA)
- Emipical formula: C5H4Cl2
- Molecular mass: 147 g/mole

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

not reported

Test solutions

Vehicle:

not specified

Details on test solutions:

Lake Superior water was sand filtered, heated.

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

- Age: 4 - 12 hours
- Life stage: embryo(s)

Study design

Test type:

not specified

Water media type:

freshwater

Remarks on exposure duration:

until hatch

Test conditions

Hardness:

44-46 mg CaCO3/L = 2.5 - 2.6 °dH

Test temperature:

25 +/- 2°C

pH:

7.3 - 7.6

Dissolved oxygen:

6.9 +/- 0.6 (5.6-8.2) mg/L

Details on test conditions:

- Live brine shrimp nauplii Artemia salina were fed two or three times daily
- The fish were not fed the last 24 hours of the test
- Chambers were cleaned as needed

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

32 d

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: growth

Effect concentrations 2

Duration:

32 d

Dose descriptor:

NOEC

Effect conc.:

2.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: until hatch

Effect concentrations 3

Duration:

32 d

Dose descriptor:

LOEC

Effect conc.:

2.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The toxicity to fish (pimephales promelas) of 1,3-dichlorobenzene was determined at a NOEC (mortality) of 2.4 mg/L until hatch, a NOEC (growth) of 1 mg/L and a LOEC (mortality) of 2.3 mg/L after 32 days.

Executive summary:

The acute toxicity to fish (Pimephales promelas) of 1,3-dichlorobenzene was determined according to a national standard method " APHA (1971): Standard Methods for the examination of water and wastewater" and showed results at a NOEC (mortality) of 2.4 mg/L until hatch, a NOEC (growth) of 1 mg/L and a LOEC (mortality) of 2.3 mg/L after 32 days.