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EC number: 208-792-1 | CAS number: 541-73-1
Biodegradation in water and sediment: simulation tests
Administrative data
- Endpoint:
- biodegradation in water and sediment: simulation tests
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Endpoint study record was created to contribute to the overall results; acceptable, well documented publication/study report which meets basic scientific principles
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Bildung von Chlorphenolen durch mikrobielle Umwandlung von Chlorbenzolen
- Author:
- Ballschmiter K, C Unglert und P Heizmann
- Year:
- 1 977
- Bibliographic source:
- Angew. Chem 89(9):680-681
- Reference Type:
- secondary source
- Title:
- m-dichlorobenzene BUA-Stoffbericht 8 (Juni 1981) [Report in German]
- Author:
- GDCh, BUA
- Year:
- 1 987
- Bibliographic source:
- publisher: VCH; ISSN: 0179-2601
Materials and methods
- Principles of method if other than guideline:
- other: biotransformation using mixed and pure cultures of bacteria
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,3-dichlorobenzene
- EC Number:
- 208-792-1
- EC Name:
- 1,3-dichlorobenzene
- Cas Number:
- 541-73-1
- Molecular formula:
- C6H4Cl2
- IUPAC Name:
- 1,3-dichlorobenzene
- Test material form:
- other: liquid
- Details on test material:
- no data
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: precultured mixed cultures of soil bacteria
- Details on inoculum:
- Were used to benzene precultured mixed cultures of soil bacteria, preferably gram-negative polar flagellated rods, which still stood in the mineral salt solution (pH = 6.8) benzene as a carbon source.
- Duration of test (contact time):
- 24 - 500 h
Initial test substance concentration
- Initial conc.:
- 20 - 200 µg/L
- Based on:
- test mat.
- Details on study design:
- 200 mg of benzene and 50 mg of 1,3-dichlorobenzne diffused layer of a hard paraffin (2 g) in 200 mL culture medium.
Here, a 0.000001 and 0.0000001 M solution of 1,3-dichlorbenzene (about 20 to 200 g / L) was established
After 24-, 100-, 240- and 500 hours incubation at 28 ° C were worked together on chlorophenols cells and nutrient medium.
Results and discussion
- Transformation products:
- yes
Identity of transformation productsopen allclose all
- No.:
- #1
Reference
- Reference substance name:
- Unnamed
- IUPAC name:
- 2,4-dichlorophenol
- Inventory number:
- InventoryMultipleMappingImpl [inventoryEntryValue=EC 204-429-6]
- CAS number:
- 120-83-2
- Identity:
- Phenol, 2,4-dichloro-
- Molecular formula:
- C6H4Cl2O
- Molecular weight:
- 163.001
- SMILES notation:
- Oc1ccc(Cl)cc1Cl
- InChl:
- InChI=1/C6H4Cl2O/c7-4-1-2-6(9)5(8)3-4/h1-3,9H
- No.:
- #2
Reference
- Reference substance name:
- Unnamed
- IUPAC name:
- 2,5-dichlorophenol
- Inventory number:
- InventoryMultipleMappingImpl [inventoryEntryValue=EC 209-520-4]
- CAS number:
- 583-78-8
- Identity:
- 2,5-dichlorophenol
- Identity:
- Phenol, 2,5-dichloro-
- Molecular formula:
- C6H4Cl2O
- Molecular weight:
- 163.001
- SMILES notation:
- Oc1cc(Cl)ccc1Cl
- InChl:
- InChI=1/C6H4Cl2O/c7-4-1-2-5(8)6(9)3-4/h1-3,9H
- No.:
- #3
Reference
- Reference substance name:
- Unnamed
- IUPAC name:
- 2,6-dichlorophenol
- Inventory number:
- InventoryMultipleMappingImpl [inventoryEntryValue=EC 201-761-3]
- CAS number:
- 87-65-0
- Identity:
- 2,6-dichlorophenol
- Identity:
- Phenol, 2,6-dichloro-
- Molecular formula:
- C6H4Cl2O
- Molecular weight:
- 163.001
- SMILES notation:
- Oc1c(Cl)cccc1Cl
- InChl:
- InChI=1/C6H4Cl2O/c7-4-2-1-3-5(8)6(4)9/h1-3,9H
Any other information on results incl. tables
The elimination of 1,3-dichlorobenzene through adsorption on activated carbon and biodegradation by film-bacteria resulted in an elimination rate of 95 ± 2 % in a column with continuous flow over two years.
In the control (only film-bacteria) was the reduction only 28 ± 32 % after adaptation.A final statement on the biodegradation of 1,3-dichlorobenzene based on these results is not possible.
The biodegradation of chlorobenzene to chlorophenol by soil bacteria in aquatic solutions is due to the action of a monooxygenase.
Applicant's summary and conclusion
Validity criteria
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The elimination of 1,3-dichlorobenzene through adsorption on activated carbon and biodegradation by film-bacteria resulted in an elimination rate of 95 ± 2 % in a column with continuous flow over two years.
In the control (only film-bacteria) was the reduction only 28 ± 32 % after adaptation.
A final statement on the biodegradation of 1,3-dichlorobenzene based on these results is not possible.
The biodegradation of chlorobenzene to chlorophenol by soil bacteria in aquatic solutions is due to the action of a monooxygenase. - Executive summary:
The elimination of 1,3-dichlorobenzene through adsorption on activated carbon and biodegradation by film-bacteria resulted in an elimination rate of 95 ± 2 %in a column with continuous flow over two years.
In the control (only film-bacteria) was the reduction only 28 ± 32 % afteradaptation.A final statement on the biodegradation of 1,3-dichlorobenzene based on these results is not possible.
The biodegradation of chlorobenzene to chlorophenol by soil bacteria in aquatic solutions is due to the action of a monooxygenase.
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