1,3-dichlorobenzene

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:

biodegradation in water and sediment: simulation tests

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Endpoint study record was created to contribute to the overall results; acceptable, well documented publication/study report which meets basic scientific principles

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Bildung von Chlorphenolen durch mikrobielle Umwandlung von Chlorbenzolen

Author:

Ballschmiter K, C Unglert und P Heizmann

Year:

1977

Bibliographic source:

Angew. Chem 89(9):680-681

Reference 2

Reference Type:

secondary source

Title:

m-dichlorobenzene BUA-Stoffbericht 8 (Juni 1981) [Report in German]

Author:

GDCh, BUA

Year:

1987

Bibliographic source:

publisher: VCH; ISSN: 0179-2601

Materials and methods

Principles of method if other than guideline:

other: biotransformation using mixed and pure cultures of bacteria

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,3-dichlorobenzene

EC Number:

208-792-1

EC Name:

1,3-dichlorobenzene

Cas Number:

541-73-1

Molecular formula:

C6H4Cl2

IUPAC Name:

1,3-dichlorobenzene

Test material form:

other: liquid

Details on test material:

no data

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: precultured mixed cultures of soil bacteria

Details on inoculum:

Were used to benzene precultured mixed cultures of soil bacteria, preferably gram-negative polar flagellated rods, which still stood in the mineral salt solution (pH = 6.8) benzene as a carbon source.

Duration of test (contact time):

24 - 500 h

Initial test substance concentration

Initial conc.:

20 - 200 µg/L

Based on:

test mat.

Details on study design:

200 mg of benzene and 50 mg of 1,3-dichlorobenzne diffused layer of a hard paraffin (2 g) in 200 mL culture medium.
Here, a 0.000001 and 0.0000001 M solution of 1,3-dichlorbenzene (about 20 to 200 g / L) was established
After 24-, 100-, 240- and 500 hours incubation at 28 ° C were worked together on chlorophenols cells and nutrient medium.

Results and discussion

Transformation products:

yes

Identity of transformation products

Identity of transformation products 1

No.:

#1

Reference

Reference substance name:

Unnamed

IUPAC name:

2,4-dichlorophenol

Inventory number:

InventoryMultipleMappingImpl [inventoryEntryValue=EC 204-429-6]

CAS number:

120-83-2

Identity:

Phenol, 2,4-dichloro-

Molecular formula:

C6H4Cl2O

Molecular weight:

163.001

SMILES notation:

Oc1ccc(Cl)cc1Cl

InChl:

InChI=1/C6H4Cl2O/c7-4-1-2-6(9)5(8)3-4/h1-3,9H

Identity of transformation products 2

No.:

#2

Reference

Reference substance name:

Unnamed

IUPAC name:

2,5-dichlorophenol

Inventory number:

InventoryMultipleMappingImpl [inventoryEntryValue=EC 209-520-4]

CAS number:

583-78-8

Identity:

2,5-dichlorophenol

Identity:

Phenol, 2,5-dichloro-

Molecular formula:

C6H4Cl2O

Molecular weight:

163.001

SMILES notation:

Oc1cc(Cl)ccc1Cl

InChl:

InChI=1/C6H4Cl2O/c7-4-1-2-5(8)6(9)3-4/h1-3,9H

Identity of transformation products 3

No.:

#3

Reference

Reference substance name:

Unnamed

IUPAC name:

2,6-dichlorophenol

Inventory number:

InventoryMultipleMappingImpl [inventoryEntryValue=EC 201-761-3]

CAS number:

87-65-0

Identity:

2,6-dichlorophenol

Identity:

Phenol, 2,6-dichloro-

Molecular formula:

C6H4Cl2O

Molecular weight:

163.001

SMILES notation:

Oc1c(Cl)cccc1Cl

InChl:

InChI=1/C6H4Cl2O/c7-4-2-1-3-5(8)6(4)9/h1-3,9H

Any other information on results incl. tables

The elimination of 1,3-dichlorobenzene through adsorption on activated carbon and biodegradation by film-bacteria resulted in an elimination rate of 95 ± 2 % in a column with continuous flow over two years.

In the control (only film-bacteria) was the reduction only 28 ± 32 % after adaptation.

A final statement on the biodegradation of 1,3-dichlorobenzene based on these results is not possible.

The biodegradation of chlorobenzene to chlorophenol by soil bacteria in aquatic solutions is due to the action of a monooxygenase.

Applicant's summary and conclusion

Validity criteria

Validity criteria fulfilled:

not specified

Conclusions:

The elimination of 1,3-dichlorobenzene through adsorption on activated carbon and biodegradation by film-bacteria resulted in an elimination rate of 95 ± 2 % in a column with continuous flow over two years.
In the control (only film-bacteria) was the reduction only 28 ± 32 % after adaptation.
A final statement on the biodegradation of 1,3-dichlorobenzene based on these results is not possible.
The biodegradation of chlorobenzene to chlorophenol by soil bacteria in aquatic solutions is due to the action of a monooxygenase.

Executive summary:

The elimination of 1,3-dichlorobenzene through adsorption on activated carbon and biodegradation by film-bacteria resulted in an elimination rate of 95 ± 2 %in a column with continuous flow over two years.

In the control (only film-bacteria) was the reduction only 28 ± 32 % afteradaptation.

A final statement on the biodegradation of 1,3-dichlorobenzene based on these results is not possible.

The biodegradation of chlorobenzene to chlorophenol by soil bacteria in aquatic solutions is due to the action of a monooxygenase.