1,3-dichlorobenzene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

1,3-dichlorobenzene

EC Number:

208-792-1

EC Name:

1,3-dichlorobenzene

Cas Number:

541-73-1

Molecular formula:

C6H4Cl2

IUPAC Name:

1,3-dichlorobenzene

Test material form:

other: colorless liquid

Details on test material:

purity: 85.1%

Test animals

Species:

rat

Strain:

other: Hoe: WISKf (SPF71)

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

nose only

Vehicle:

air

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

5 m+ 5 f rats: 14.8 mg m-dichlorobenze/l air
5 m+ 5 f rats: 17.63 mg m-dichlorobenze/l air

No. of animals per sex per dose:

5 m+ 5 f rats: 14.8 mg m-dichlorobenze/l air
5 m+ 5 f rats: 17.63 mg m-dichlorobenze/l air

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 17.6 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Any other information on results incl. tables

After an expostion to 17.6 mg m-dichlorobenzene no of the animals died. The test atmosphere consited maily as vapor.

Based on this result, the LC50(4h) was > 17.6 mg dichlorobenzene/l.

Of the animals exposed to 14.8 mg m-dichlorobenene 2 male and 3 female rats died. The test atmosphere had a higher content of aerosol compared with the test group which received 17.6 mg m-dichlorobenzene/l air.

The animals exposed to 17.6 mg m-dichlorobenzene/l air showed unregular breathing, uncoordinated gait, ataxia, prone position, rough fur, drowsiness, tremor, constricted palpebral fissures, reduced startled reflexes, increased salivation and foamy nose effluent.

Animals which received furthermore reduced breathing rate, staggared gait, reducted activity, narcosis, coma, no startled reflexes, bloody nose and eye lids, closed palpebral fissures, reduced body temperature, convulsions, cyanosis bad nutrition state and sneezing.

The section of animals which died intercurrent had darkred spots on the lungs, darkened liver and blood engorgement in the lungs. The animals which were killed after the post-observation period revealed no findings.

Applicant's summary and conclusion

Interpretation of results:

other:

Remarks:

LC50(4h) > 17.6 mg m-dichlorobenzene (vapour)/l air.

Executive summary:

Five male and five female Wistar rats were exposed nose-only for 4 hours to concentrations of 14.8 or 17.63 mg dichlorobenzene/liter air. Animals were observed for mortality, body weight and clinical signs during a post-observation period of 14 days. A necropsy was performed on the animals at the end of the post-observation period.

The LC50(4h) was > 17.6 mg m-dichlorobenzene (vapour)/l air.