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Diss Factsheets
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EC number: 208-792-1 | CAS number: 541-73-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1982-07-05 till 1983-09-07
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
- Deviations:
- no
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,3-dichlorobenzene
- EC Number:
- 208-792-1
- EC Name:
- 1,3-dichlorobenzene
- Cas Number:
- 541-73-1
- Molecular formula:
- C6H4Cl2
- IUPAC Name:
- 1,3-dichlorobenzene
- Test material form:
- other: liquid
- Details on test material:
- - Purity: 98 % (according from the sponsor)
- Empirical formula: C6H4Cl2
- Molecular mass: 147.0 g/mole
Constituent 1
Sampling and analysis
- Details on sampling:
- - Concentrations: The first was 100 µg/L and the second was 10 µg/L of 1,3-dichlorobenzene.
The test water analysis was perfomed twice a week (n=1).
The fish analysis was performed every two weeks (n=2).
The control fish analysis was performed before the initiation and the termination of exposure.
Test solutions
- Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- Farming conditions:
- To remove some of the abnormal by the visual observation when fish availability, Acceptance tank in medicine Yokugo, I was 1-2 months bred in running water state.
- Desinfection: For external disinfection, the fish were placed in an aqueous solution (7 g/L sodium chloride) in a water-stop state for 24
hours.
- Acclimation: at 25 ° C for 14 days
- Stock solution: 0.1 g of 1,3-dichlorobenzene was dissolved in n-hexane to 100 mL (1000 mg/L)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween-80 (Polyoxyethylensorbitanmonooleat; 48 h LC50 = 500000 mg/L)
- Concentration of vehicle in test medium: 1000 mg/L
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- - Name: Carp (Cyprinus carpio)
- Supplier: Sugishima fish farm
- Conditions for fish keeping: Period etc.
At reception, fish showing visible abnormal signs were removed. Then fish were reared according to flow through system for 1-2 months after external
disinfection.
- External disinfection
External disinfection was performed according to static condition for 24 hours in an aqueous solution containing 50 mg/L Terramycin (Taito Pfizer) and 7 g/L sodium chloride.
- Condition of acclimation: Fish were reared in an acclimation tank according to flow through system at temperature of 25 + 2 °C for about 28 days. During the period, abnormal fish were removed. Then the fish were transfered to test tanks and reared again at the same temperature according to flow through system for about one month.
- Weight, length and lipid content.at the initiation of exposure: Weight = 32.2 g, Length = 10.7 cm, Lipid content = 4.8 %
- Feeding:
Feed = Pelleted feed for carp
Manufacturer = Japan Haigo Shiryo K.K.
Feeding amount and interval. Amount corresponding to about 2 % of the total body weight of test fish was fed twice a day by halves.
On the day of fish sampling feed.was not supplied.
Study design
- Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 8 wk
Test conditions
- Hardness:
- not specified
- Test temperature:
- 25 °C
- pH:
- not specified
- Dissolved oxygen:
- 6-8 mg/L
- Details on test conditions:
- - Temperature: 25 ± 2 °C; Temperature controlled test water was supplied continuously into the flow through system (Aquatron).
- Test tank: Glass tank of 100 liters in volume, exchange frequency 1-2 times per month
- Flow rate of test water: At a rate of 800 L/day of test water was supplied.
- Number of fish (at the initiation of exposure): 25 fish/Glass tank
- During the exposure: 8 weeks
- Feeding: Food was shared, 2 times per day, 2 % of fish body weight
- Photoperiod: 14 h light / 10 h dark - Nominal and measured concentrations:
- nominal concentrations: first = 100 µg/L and second = 10 µg/L
- Reference substance (positive control):
- no
- Details on estimation of bioconcentration:
- BASIS INFORMATION
- Measured logPow: 3.72
BASIS FOR CALCULATION OF BCF
BCF = concentration of test substance in fish/concentration of test substance in water
Results and discussion
Lipid content
- Lipid content:
- 4.8 %
- Time point:
- end of exposure
Bioaccumulation factoropen allclose all
- Type:
- BCF
- Value:
- 58 - 370
- Remarks on result:
- other: Conc.in environment / dose:10 µg/L
- Type:
- BCF
- Value:
- 57 - 229
- Remarks on result:
- other: Conc.in environment / dose:100 µg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Remarks:
- (expected to be a valid source)
- Conclusions:
- The experimental BCF values in carp (Cyprinus carpio) after 8 weeks of exposure are 57 - 229 (concentration 100 µg/L) and 58 - 370
(concentration 10 µg/L) for 1,3-dichlorobenzene. 1,3-dichlorobenzene should be considered to "Not Bioaccumulative in Aquatic Organisms" according to the EC regulation no 1272/2008. - Executive summary:
The determination of the bioconcentration of 1,3-dichlorobenzene was tested according to the guideline "Bioaccumulation test of chemical substances in fish and shellfish" (1974) and corresponding to OECD guideline 305C (Bioaccumulation: Test for the degree of bioconcentration in Fish, 1981). They exposed Cyprinus carpio (carp) with a lipid content of 4.8 % to 100 µg/L and 10 µg/L of 1,3 -dichlorobenzene during 8 weeks. After this period they obtained experimental BCF values of 57 - 229 (concentration 100 µg/L) and 58 - 370 (concentration 10 µg/L). 1,3 -dichlorobenzene should be considered to "Not Bioaccumulative in Aquatic Organisms" according to the EC regulation no 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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