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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is not available and has not been reviewed.

Data source

Referenceopen allclose all

Reference Type:
review article or handbook
Title:
1,4-Dichlorobut-2-ene- CAS No: 764-41-0
Author:
OECD SIDS
Year:
2006
Bibliographic source:
SIDS Initial Assessment Report for 22th SIAM, UNEP Publications
Reference Type:
study report
Title:
Unnamed
Year:
1974
Report Date:
1974

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The LD50 of a mixture of 1.43 % cis- and 97.17% trans-isomer has been determined in ChR-CD rats after a 30 minutes exposure.
GLP compliance:
no
Remarks:
GLP was not mandatory at the time of the study
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
1.43 % cis- and 97.17% trans-1,4-dichlorobut-2-ene.

Test animals

Species:
rat
Strain:
other: ChR-CD
Sex:
male

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: filtered air
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
30 min
Concentrations:
240/370/410/440/540/760/2600 ppm
No. of animals per sex per dose:
6 (only male rats)
Control animals:
other: not required
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, histopathology, gross examination. Gross and histopathologic examinations were performed on 2 rats surviving exposure for 7 days, on 1 rat found dead after 13 recovery days and on 1 rat after 14 recovery days.
Statistics:
LC50 calculations were made by a computerized version of the probit method of Finney (1964).

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
4.1 mg/L air
Based on:
test mat.
Exp. duration:
30 min
Mortality:
Mortality %: 0/0/33/0/0 /83/100
Clinical signs:
other: Clinical signs were observed already in the lowest dose group (240 ppm; 1248 mg/m³): Inactivity, closed eyes, shallow respiration, pale ears, salivation, lacrimation, wet fur and reversible loss of body weight. Clinical signs at 760 ppm: Irregular respira
Body weight:
No data
Gross pathology:
No data
Other findings:
- Histopathology: at 410 ppm and above: damage of the tracheobronchial epithelium was observed. Furthermore in animals exposed to 760 ppm (3950 mg/m³) there was kidney damage, testicular atrophy and hypoplastic bone marrow observed. These effects have been interpreted by the authors of the study as representing only a reflection of stress and emaciation and thus being not directly compound-related. The other tissues examined (lymph node, stomach, duodenum, epididymis, thyroid, adrenal gland, brain, eye) revealed no effects being attributable to the test substance.

Applicant's summary and conclusion