Reaction mass of 1,4-dichlorobut-2-ene; 3,4-dichlorobut-1-ene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is available and has been reviewed.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1959

Report date:

1959

Reference 2

Reference Type:

secondary source

Title:

1,4-Dichlorobut-2-ene- CAS: 764-41-0

Author:

OECD SIDS

Year:

2006

Bibliographic source:

SIDS Initial Assessment Report for 22th SIAM, UNEP Publications

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

In an acute oral toxicity study on male albino rats (3-5 animals/group) the LD50 has been determined.

GLP compliance:

no

Remarks:

GLP was not mandatory at the time of the study

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dichlorobut-2-ene

EC Number:

212-121-8

EC Name:

1,4-dichlorobut-2-ene

Cas Number:

764-41-0

Molecular formula:

C4H6Cl2

IUPAC Name:

1,4-dichlorobut-2-ene

Details on test material:

- Name of test material (as cited in study report): 1,4-dichlorobut-2-ene.
- Analytical purity: no data

Test animals

Species:

rat

Strain:

other: albino

Sex:

male

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 0.05, 0.10, 0.25, 0.50 mL/kg bw
- Amount of vehicle (if gavage): between 1180-1190 mL
- Justification for choice of vehicle: test substance undergoes hydrolysis in water

MAXIMUM DOSE VOLUME APPLIED: between 1180-1190 mL

Doses:

59, 119, 297, 593 mg/kg bw

No. of animals per sex per dose:

3-5

Control animals:

other: not necessary

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

> 120 - 300 mg/kg bw

Effect levels 2

Sex:

male

Dose descriptor:

other: NOAEL

Effect level:

59 mg/kg bw

Effect levels 3

Sex:

male

Dose descriptor:

other: LOAEL

Effect level:

119 mg/kg bw

Remarks on result:

other: LOAEL yield a mortality of 20 % (1/5 animals) and toxic symptoms like spasms, inappetence and weakness were observed in all treated animals. At 297 mg/kg bw 66% mortality (2/3 animals) was observed.

Mortality:

59 mg/kg bw (0.05 mL/kg bw 0.5 % solution in oil): no mortality NOAEL (n= 3);
119 mg/kg bw (0.10 mL/kg bw 1.0 % solution in oil): mortality 1/5;
297 mg/kg bw (0.25 mL/kg bw 2.5 % solution in oil): mortality 2/3;
593 mg/kg bw (0.50 mL/kg bw 5.0 % solution in oil): mortality 3/3.

Clinical signs:

119 mg/kg bw (0.10 mL/kg bw 1.0 % solution in oil): all animals with toxic symptoms (spasms, inappetence, weakness).
297 mg/kg bw (0.25 mL/kg bw 2.5 % solution in oil): all animals with toxic symptoms (spasms, inappetence, weakness).
593 mg/kg bw (0.50 mL/kg bw 5.0 % solution in oil): all animals with toxic symptoms (spasms, inappetence, weakness).

Body weight:

no data

Gross pathology:

no data