Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2004
Reference Type:
secondary source
Title:
1,4-Dichlorobut-2-ene - CAS No: 764-41-0
Author:
OECD SIDS
Year:
2006
Bibliographic source:
SIDS Initial Assessment Report for 22th SIAM, UNEP Publications

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Principles of method if other than guideline:
EU Method C.3 is in most parts equivalent to the OECD TG 201
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
IUCLID4 Test substance: other TS: 1,4-Dichloro-2-butene, purity 96.1% (31.9% cis- and 64.2% trans-isomer)

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
72 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
5.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL: 5.0-5.6 mg/L
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
1.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
3.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
2.13 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.68 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
1.35 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

All results are expressed in terms of mean measured concentrations. The recovery rate of the test substance ranged from 57 -83 % of nominal values in the freshly prepared media at 0 hours, and from 2 - 3 % of nominal values in the media after 72 hours of exposure, respectively. Therefore, in contrast to the original study in the OECD SIDS (2006) the effect concentrations (EC50, NOEC, LOEC) are calculated on the basis of the geometric mean.

Applicant's summary and conclusion