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EC number: 248-299-9 | CAS number: 27178-16-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions, no GLP, no analytical monitoring
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- The study was performed according to European standard test guideline, Directive 79/831/EWG, appendix V, part C, stand May 1988.
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- - concentration of the stock solution: 200 mg/l
- solubilized in 40 mg/l Cremophor RH 40
- range of test concentration: 0.78 - 100 mg/l - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISMS
- Test organism: Scenedesmus subspicatus CHODAT
- Supplier: Culture Collection of Algae Goettingen, Germany
CULTIVATION
- temperature: 23°C
- photoperiod: permanent lighted
- cultivation water was identical to test water
- stock culture 10000 cells/ml
- cultivation in 250 ml Erlenmeyer flasks - Water media type:
- freshwater
- Limit test:
- no
- Nominal and measured concentrations:
- - test concentrations nominal (mg/l): 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 100
- Details on test conditions:
- TEST CONDITIONS
- 8 ml test volume filled in glass vessels
- 4 replicates
- 2 control vessels
- test duration 72 hours
- fluorometric measurement after 0, 24, 48 and 72 hours (wave length 435 nm)
- pH control at the end of the test - Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 - 27 Oct 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412, part 9
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A 500 mg/L stock solution was preparted using 200 mg/L Cremophor as dispersant. After stirring of 17h the test solution was still an emulsion. This emulsion was tested.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Cremophor
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 200 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Test solutions were emulsions. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISMS
- Strain: SAG 86.81
- Method of cultivation: temperature: 20°C, stock culture 10000 cells/mL, cultivation in 250 ml Erlenmeyer flasks, test volume 100 mL - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 20 °C
- pH:
- not specified
- Nominal and measured concentrations:
- Nominal: 7.812, 15.625, 31.25, 62.5, 125, 250 and 500 mg/L
- Details on test conditions:
- TEST SYSTEM
- Fill volume: 10 mL
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
GROWTH MEDIUM
- Standard medium used: yes (OECD medium)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not specified
- Culture medium different from test medium: no
EFFECT PARAMETERS MEASURED: Biomass was recorded after 0, 24, 48 and 72h exposure time.
- Determination of cell concentrations: fluorimeter - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 398.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no - Validity criteria fulfilled:
- yes
- Conclusions:
- A 72h acute toxicity test with Scenedesmus subspicatus revealed that DEHA is not acutely harmful to freshwater algae up to the saturation limit in water.
- Executive summary:
The study was performed according to DIN guideline 38412, part 9 using Scenedesmus subspicatus as test organism. A 500 mg/L stock solution was preparted using 200 mg/L Cremophor as dispersant. After stirring of 17h the test solution was still an emulsion. This emulsion was tested. Nominal test concentrations between 7.8 and 500 mg/L were tested. The 72h-EC50 value is determined to be > 500 mg/L. An 72h-EC20 value is determined to be 398.6 mg/L. Since the effect concentration is greater than the solubility of DEHA in the test medium, it can be concluded that DEHA is not harmful to freshwater algae up to the saturation limit in water.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- Please see Section 13 below.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 398.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- Please see Section 13 below.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Referenceopen allclose all
RESULT GROWTH RATE
ErC 10 (72h): 72 mg/l
ErC 50 (72h): > 100 mg/l
ErC 90 (72h): > 100 mg/l
RESULT BIOMASS
EbC 10 (72h): 14 mg/l
EbC 50 (72h): > 100 mg/l
EbC 90 (72h): > 100 mg/l
REMARK
Effect concentration is greater than the solubility of the substance in the test medium.
Description of key information
No relevant studies are available for diisodecyl adipate.
Two long-term Daphnia toxicity studies are available for closely related, read-across substances:
The study with read-across substance, bis(2-ethylhexyl) adipate (CAS 103-23-1, DEHA), was performed according to DIN guideline 38412, part 9 using Scenedesmus subspicatus as test organism. A 500 mg/L stock solution was prepared using 200 mg/L Cremophor as dispersant. After stirring of 17h the test solution was still an emulsion. This emulsion was tested. Nominal test concentrations between 7.8 and 500 mg/L were tested. The 72h-EC50 value is determined to be > 500 mg/L. A 72h-EC20 value is determined to be 398.6 mg/L. Since the effect concentration is greater than the solubility of DEHA in the test medium, it can be concluded that DEHA is not harmful to freshwater algae up to the saturation limit in water.
The test with Diisononyl adipate (CAS 33703-08-1) was conducted under static conditions similar to OECD 211 using Desmodesmus subspicatus as test organism (BASF, 1990).Cremophor RH 40 was used as vehicle at a concentration of 100 mg/L. Test concentrations of 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L were applied.The EC50/EC10 (72h) was calculated as > 100/72 mg/L (nominal), based on growth rate.
Key value for chemical safety assessment
Additional information
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