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EC number: 248-299-9 | CAS number: 27178-16-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- Please see Section 13 below.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- > 90
- Sampling time:
- 28 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Read-across study
- Justification for type of information:
- Please see Section 13 below.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- >= 90 - <= 100
- Sampling time:
- 28 d
- Key result
- Parameter:
- COD
- Value:
- 1 687 mg O2/g test mat.
- Key result
- Parameter:
- BOD5
- Value:
- 277 g O2/g test mat.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Principles of method if other than guideline:
- aerobic guideline: EEC Directive 79-831 Annex V, Part C, Manometric Respirometry Year: Bruessel 1984
Remark: Method is comparable to OECD guideline 301 F and ISO 9408 - GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Activated sludge from laboratory waste water plant Oppau
- Concentration of dry substance in the test = 30 mg/l
- 10 ml aliquot of sludge added per test - Duration of test (contact time):
- 28 d
- Initial conc.:
- 200 mg/L
- Based on:
- test mat.
- Initial conc.:
- 50 mg/L
- Based on:
- test mat.
- Details on study design:
- - stock solution: 759 mg/g DOC
- concentration of test substance: 50, 100, 200 mg/l - Reference substance:
- aniline
- Parameter:
- % degradation (O2 consumption)
- Value:
- > 90
- Sampling time:
- 28 d
- Details on results:
- RESULT REFERENCE SUBSTANCE
- Reference substance: aniline
- degradation phase 2 days
- DOC elimination 96 %
- BOD/ThOD = 75%
- lag phase 4 days
RESULT TEST SUBSTANCE
- lag phase 1 day
- degradation phase 4 days
- biodegradation degree after 28 days: >90%
- the test substance was readily biodegradable - Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 Mar - 14 Apr 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- adopted in 1984
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: obtained from municipal laboratory wastewater treatment plant (16 Mar 1987)
- Concentration of sludge: 30 mg/L (in test) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 52 - 200 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Number of culture flasks/concentration: 100 mg/L: 3 replicats; 52/200 mg/L: two replicats
- pH: 6.8 - 7.8
CONTROL AND BLANK SYSTEM
- Toxicity control: yes - Reference substance:
- aniline
- Remarks:
- 100 mg/L
- Parameter:
- % degradation (O2 consumption)
- Value:
- 90 - 100
- Sampling time:
- 28 d
- Details on results:
- - lag phase 3 day
- > Manometric Respirometry test60% degradation after 10 days
- biodegradation degree after 28 days: >90%
The test substance is readily biodegradable. - Parameter:
- COD
- Value:
- 1 687 mg O2/g test mat.
- Parameter:
- BOD5
- Value:
- 277 mg O2/g test mat.
- Results with reference substance:
- 100% DOC elimination in 10 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance reached a biodegradation of 90 - 100% in 28 days. The 10-day-window was passed. Therefore, the test substance is readily biodegradable according to OECD criteria.
- Executive summary:
The ready biodegradability of Bis(2-ethylhexyl) adipate was tested according to EU method C.4-D (manometric respirometry test equivalent to OECD 301F) under GLP conditions. Non-adapted municipal activated sludge was used as inoculum. The test substance was tested at concentrations between of 52 and 200 mg/L. The validity criteria are fulfilled. The pass level of 60% biodegradation was reached in less than 10 days. After 28 days a biodegradation of 90 – 100 % was observed. Therefore, the test substance is readily biodegradable according to OECD criteria.
Referenceopen allclose all
The toxicity control indicates that the test substance is not inhibitory to activated sludge microorganisms (> 90% degradation after 14 days)
Description of key information
Read-across substance, bis(2-ethylhexyl) adipate, was determined to be readily biodegradable.
Read-across substance, diisononyl adipate, was determined to be readily biodegradable.
Read-across substance, ditridecyl adipate, was determined to be readily biodegradable.
Read-across substance, bis(6 -methylheptyl) adipate, was determined to be readily biodegradable.
Therefore, based on read-across from these four similar substances, diisodecyl adipate is considered to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
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