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Diss Factsheets
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EC number: 231-391-8 | CAS number: 7529-22-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 June 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: yes, municipal sludge taken from a laboratory wastewater treatment fed with municipal sewage
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated for 24 hours.
50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Test temperature:
- 20 ± 2°C
- Dissolved oxygen:
- Oxygen concentration during aeration: > 2.5 mg/L
Oxygen concentration immediately before measurement: > 6.5 mg/L - Nominal and measured concentrations:
- Nominal: 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel
- Material, size, headspace, fill volume: glass, nominal volume: 250 mL, fill volume 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium
OTHER TEST CONDITIONS
- Adjustment of pH: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? yes. EC50 of 3,5-dichlorophenol is in the range of 5-30 mg/L
- Relevant effect levels: 30 min EC20 = ca. 3.0 mg/L; 30-min EC50 = ca 15 mg/L; 30-min EC80 = ca. 50 mg/L (highest concentration tested: 100 mg/L) - Validity criteria fulfilled:
- yes
- Conclusions:
- No inhibition of respiration was measured at the concentration tested (1000 mg/L).
- Executive summary:
The inhibitory effect of N-methylmorpholine oxide on activated sludge respiration was tested according to OECD guideline 209 (version 1999) during an exposure time of 30 min. One replicate of 1000 mg/L test substance was used in a limit test. The final concentration of solids in the test vessels was 1 g/L. Inhibition was not observed, therefore the NOEC is reported to be >= 1000 mg/L and the EC20 is stated to be >1000 mg/L.
The study was valid at the time performed.
Reference
Measured data - blank control (BC) and test substance (TS) after 30 minutes incubation time
BC1 |
BC2 |
BC3 |
mv BC |
TS1 |
|
Concentration of test substance (mg/L) |
- |
- |
- |
- |
1001 |
Added volume of stock solution (mL/vessel) |
- |
- |
- |
- |
125.0 |
Direct addition (mg/vessel) |
- |
- |
- |
- |
- |
Oxygen concentration start (mg O2/L) |
7.8 |
8.1 |
7.9 |
- |
8.1 |
Oxygen concentration after 6 min (mg O2/L) |
6.2 |
6.6 |
6.2 |
- |
6.5 |
Oxygen consumption rate (mg O2/L * 6 min) |
1.6 |
1.5 |
1.7 |
1.6 |
1.6 |
Oxygen consumption rate (mg O2/L*h) |
16 |
15 |
17 |
16 |
16 |
Specific oxygen consumption rate ( mg O2/g*h) |
16 |
15 |
17 |
16 |
16 |
Change in oxygen consumption rate compared with the blank control (%) |
- |
- |
- |
- |
0 |
mv BC = mean value of blank control
pH-values - blank control (BC) and test substance (TS) after 30 minutes incubation time
Vessel no.: |
BC1 |
BC2 |
BC3 |
TS1 |
pH-value before adding the inoculum before correction: after correction: |
6.2 7.3 |
6.2 7.3 |
6.2 7.4 |
6.3 7.4 |
pH-value after an incubation time of 30 min |
- |
- |
- |
7.4 |
Measured data - reference substance (RS) after 30 minutes incubation time
Vessel no.: |
mv BC |
RS1 |
RS2 |
RS3 |
Concentration of test substance (mg/L) |
- |
1 |
10 |
100 |
Added volume of stock solution (mL/vessel) |
- |
0.5 |
5.0 |
50.0 |
Oxygen concentration start (mg O2/L) |
- |
7.8 |
8.3 |
8.7 |
Oxygen concentration after 6 min (mg O2/L) |
- |
6.3 |
7.3 |
8.7 |
Oxygen consumption rate (mg O2/L * 6 min) |
1.6 |
1.5 |
1.0 |
0.0 |
Oxygen consumption rate (mg O2/L*h) |
16 |
15 |
10 |
0 |
Specific oxygen consumption rate ( mg O2/g*h) |
16 |
15 |
10 |
0 |
Change in oxygen consumption rate compared with the blank control (%) |
- |
6 |
37 |
100 |
pH-values - reference substance (RS) after 30 minutes incubation time
Vessel no.: |
RS1 |
RS2 |
RS3 |
pH-value before adding the inoculum before correction: after correction: |
6.2 7.2 |
6.2 7.2 |
6.4 7.2 |
Description of key information
There is one study from BASF available. This result was chosen to derive PNEC-STP . The respiration inhibition test from BASF was performed according to a guideline (OECD 209) valid at that time including a test duration of 30 min. The 30 min. EC20 value of > 1000 mg/L is used for the CSA and derivation of the PNEC.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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