Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-391-8 | CAS number: 7529-22-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-01-12 to 2012-04-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Department of Health of the Government of the United Kingdom
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:all, at 0 and 48 hr
- Sample storage conditions before analysis: -20 degrees C until analysed - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dilution in test medium; serial dilution for concentrations.
- Controls: Test medium without test substance added
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Dapnia magna
- Source: in-house culture
- Age at study initiation (mean and range, SD): <24 hr
- Method of breeding: parthenogenesis
- Feeding during test: no
ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
- Hardness:
- 250 mg/L CaCO3
- Test temperature:
- 21-22 degrees C
- pH:
- 7.7-8.0 SU
- Dissolved oxygen:
- Saturated
- Nominal and measured concentrations:
- Nominal- Control and 100 mg/L test substance
Measured concentrations were 96-102 % of nominal. - Details on test conditions:
- TEST SYSTEM
- Test vessel: jar
- Type (delete if not applicable): closed; covered
- Material, size, headspace, fill volume: 250 mL glass, 200mL test volume
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted lab water used to make Elendt M7 standard Medium
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:16:8 light:dark, with 20 minute dawn and dusk transitions
- Light intensity:662-723 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility, every 24 h
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0.10, 1.0 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: yes
- EC50/LC50: 1.1 mg/L, 95% CL 1.0-1.3 mg/L
- Other: results within normal ranges - Reported statistics and error estimates:
- None- EC50 estimated by visual inspection
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 of the test substance to D. magna was found to be > 100 mg/L test substance.
- Executive summary:
The 48-h toxicity of N-Methylmorpholine oxide to Daphnia magna was studied in an OECD 202 guideline study under static conditions. The study was conducted as limit test with a control ( 0 mg/L) and at 100 mg/L test substance (nominal concentration). No effects regarding immobilisation were observed. Therefore, the 48 h NOEC was >= 100 mg/L and the 48 h EC50 was > 100 mg/L.
The study is classified as acceptable and satisfies guideline requirements for a acute toxicity study with freshwater invertebrates.
Results synopsis:
Test organism age: <24h
Test type: static
EC50 > 100 mg/L
NOEC: >= 100 mg/L
LOEC: > 100 mg/L
Endpoint(s) assessed: immobilisation
Reference
Description of key information
The 48-h toxicity of N-Methylmorpholine oxide to Daphnia magna was studied in an OECD 202 guideline study under static conditions. The study was conducted as limit test with a control ( 0 mg/L) and at 100 mg/L test substance (nominal concentration). No effects regarding immobilisation were observed. Therefore, the 48 h NOEC was >= 100 mg/L and the 48 h EC50 was > 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Short-term toxicity of the test substance to invertebrates was investigated by exposing Daphnia magna (waterflea) during 48 hours in a static test, according to the OECD guideline 202. This resulted in an unbounded EC50 value of > 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
