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relative density
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
EU Method A.3 (Relative Density)
GLP compliance:
yes (incl. QA statement)
Type of method:
pycnometer method
Key result
relative density
1.135 other: (corresponds to D4(20))
20 °C

Determining the density of the immersion liquid:

 Run number  1
 Mass of empty pycnometer (g)  20.92
 Temperature (°C)  20
 Mass of water and pycnometer (g)  45.78
 Mass of immersion liquid and pycnometer (g)  45.00
 Calculated Density of water using equation 1 (g/cm3)  0.994
 Calculated Density of immersion liquid using equation 2 (g/cm3)  0.963
 Density of immersion liquid (kg/m3)  963

Equation 1 (Calculation for the density of water)

Mass of water/volume of pycnometer = density of water

Equation 2 (Calculation of density of immersion liquid)

rhoIL = (mIL/mW) x rhow,t


mIL is the mass of immersion liquid

mw is the mass of water

rhow,t is the density of water at temperature t

Calculated value for the density of immersion liquid = 963 kg/m3 at 20°C

Determining the density of the sample:

 Run Number  1  2
 Mass of Empty Pycnometer (g)  20.92  20.92
 Mass of Sample & Pycnometer (g) 31.14  30.10 
Mass of Sample added to empty Pycnometer (g)  10.22 9.18 
 Mass of Sample, Pycnometer & immersion liquid  46.70 46.24 
 Mass of immersion liquid added (g)  15.56  16.14
 Calculated Density of Sample (g/cm3) 1.155  1.114 
 Density of Sample (kg/m3) 1155  1114 
 Mean result (kg/m3) 1135


Equation 3 (Calculation for the density of sample)

rho s,t = m s rho IL/m1 - m2

Where m s is the mass, in grams, of the sample:

m1 is the mass, in grams, of immersion liquid required to fill the pycnometer;

m2 is the mass, in grams, of immersion liquid required to fill the pycnometer containing the specimen;

rho IL is the density of the immersion liquid (as determined by equation 1)

The relative density of the substance was determined to be 1.135 at 20 °C.
Executive summary:

The relative density of the test item was determined according to EU Method A.3. by applying the pycnometer method. The relative density of the substance was determined to be 1.135 at 20 °C.

Description of key information

The relative density of NMMO was determined in a GLP study according to the EU method A.3 (relative density) to be 1.135 at 20°C, following the pycnometer method (Younis, 2012).

Key value for chemical safety assessment

Relative density at 20C:

Additional information