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EC number: 215-202-6 | CAS number: 1313-13-9
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
No toxic effects at the limit of solubility of the test material in the test meidum, OECD 202, EU Method C.2, Goodband & Mullee (2010)
Key value for chemical safety assessment
Additional information
The short term toxicity of the test material to aquatic invertebrates was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 202 and EU Method C.2.
As the test material is considered to be poorly soluble in water the study was conducted with a saturated solution of test material.
Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test material at a concentration of 100% v/v saturated solution for 48 hours at a temperature of approximately 20°C under static test conditions. The test material solution was prepared by stirring an excess (100 mg/L) of test material in reconstituted water using a magnetic stirrer at approximately 100 rpm for 48 hours. After the stirring period any undissolved test material was removed by filtration through a 0.2 µm filter to give a saturated solution of the test material. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours.
A positive control conducted approximately every six months used potassium dichromate as the reference item. Daphnia magna were exposed to an aqueous solution of the reference item at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L for 48 hours at a temperature of approximately 20°C under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours.
No daphnia immobilisation was observed over the 48 hour test period. The 48-hour EC50 was therefore determined to be greater than 100% v/v saturated solution. This study showed that there were no toxic effects at the limit of solubility of the test material in the test medium.
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