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EC number: 215-202-6 | CAS number: 1313-13-9
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
No toxic effects up to the limit of solubility of the test material in the test medium, OECD 203, EU Method C.1, Goodband & Mullee (2010)
Key value for chemical safety assessment
Additional information
The acute toxicity of the test material to fish was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 203 and EU Method C.1.
As the test material is considered to be poorly soluble in water, a saturated solution of test material was prepared for use in the study.
Following a preliminary range-finding test fish were exposed, in two groups of seven, to an aqueous solution of the test material, at a single concentration of 100% v/v saturated solution for a period of 96 hours at a temperature of approximately 14ºC under semi-static test conditions. The test material solution was prepared by stirring an excess (100 mg/L) of test material in dechlorinated tap water using a magnetic stirrer at approximately 100 rpm at a temperature of approximately 14°C for 48 hours. After the stirring period any undissolved test material was removed by filtration through a 0.2 µm filter to give a saturated solution of the test material.
The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.
Two fish were observed dead in the 100% v/v saturated solution Replicate R2 after 72 hours exposure. Given that no other mortalities were observed, and no sub-lethal effects were observed throughout the duration of the test, these mortalities were observed to be due to natural causes rather than a toxic effect of the test material. There were no sub-lethal effects of exposure observed in 14 fish exposed to a test concentration of 100% v/v saturated solution for a period of 96 hours.The 96-hour LC50 value was therefore considered to be greater than 100% v/v saturated solution.
Therefore, under the conditions of the study there were no toxic effects up to the limit of solubility of the test material in the test medium.
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