Ethylene dinitrate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Test conducted according to ASTM Designation E 729-80

Deviations:

not specified

GLP compliance:

no

Remarks:

Test method is fully documented. Uncertain whether method is fully consistent with GLP (not compared in detail). However, the method was a national method based on sound scientific principles.

Test material

Test material information

Constituent 1

Reference substance name:

Glycerol trinitrate

EC Number:

200-240-8

EC Name:

Glycerol trinitrate

Cas Number:

55-63-0

Molecular formula:

C3H5N3O9

IUPAC Name:

propane-1,2,3-triyl trinitrate

Specific details on test material used for the study:

NG dissolved in JHU/APL well water at 1g/L was obtained from the US Naval Ordnance Section, Indian Head, MD.
Reverse phase HPLC determined that no impurities were present during testing.
Stability of stock solution was checked periodically during the study period.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

Test species was fathead minnow (Pimephales promelas) juveniles. They were obtained from the JHU/APL culture maintained at 25 °C.
Fry were reared on bring shrimp nauplii in 19 L aquaria for 20 d.
Feeding was discontinued 24 h prior to test initiation.
10 fish were randomly assigned to each control or treatment replicate.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Raw data are presented for each replicate.
Mortality observations were made every 24 h.

Test conditions

Details on test conditions:

Flow through system.
Acute test
Negative control with 2 replicates was run concurrently.
2 replicates per treatment
Exposure period was 96 h
Exposure pathway was aqueous
Geometric dilution series of 5 concentrations was tested with the intent that two of the dilutions would produce 84% survival and 84% mortality, respectively.

Photoperiod, temperature, DO, pH, alkalinity, conductivity and total hardness were monitored and reported.
Photoperiod was 16:8 light:dark.
Mean and range of temperature and water quality parameters fell within ranges specified in study guideline.

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3.58 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)