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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Sodium diisobutylnaphthalenesulphonate
EC Number:
248-326-4
EC Name:
Sodium diisobutylnaphthalenesulphonate
Cas Number:
27213-90-7
Molecular formula:
Not applicable (UVCB substance)
IUPAC Name:
sodium 2,3-bis(2-methylpropyl)naphthalene-1-sulfonate
Test material form:
solid: particulate/powder

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 - < 220 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

The purpose of this study was to determine the acute toxicity of test item on the Zebra fish (Brachydanio rerio HAM. and BUCH.) using a static procedure. Zebra fish were exposed aver a 96-hour period to a range of 5 concentrations spaced by a factor of about 2.2: 46.4, 100. 215, 464 and 1000 mg/l.

An untreated control was included. The study procedure was based an the OECD guideline.

Results (on the basis of the nominal concentrations):

LC 50 after 96 hours

GREATER 100 (MG/L)(1% SIGNIFICANCE LEVEL)

SMALLER 220 (MG/L)(1% SIGNIFICANCE LEVEL)

Symptoms:

apathy

No observed effect concentration (NOEC): 100 mg/l

Maximum concentration tested causing no mortality: 100 mg/l

Minimum concentration tested causing 100% mortality: 215 mg/l

 

Results (on the basis of the measured concentrations):

Values after 96 hours

LC 50:

GREATER 100 <MG/L) ( 1% SIGNIFICANCE LEVEL)

SMALLER 220 <MG/L) ( 1% SIGMIFICANCE LEVEL)

Na observed effect concentration (NOEC): 100 mg/l

Maximum concentration tested causing no mortality: 100 mg/l

Minimum concentration tested causing 100% mortality: 216 mg/l

The analytically detected concentrations were initially in the range of 99.5% - 105.6% and after about 96 hours between 100.0% - 103.4% of the nominal concentrations

Applicant's summary and conclusion

Conclusions:
Based on the nominal concentrations the median lethal concentration LC 50 <96 h) is > 100 < 220 mg/l.
Mortality (10/10) occurred from the concentration 215 mg/l upward.
Another substance-related effect was the symptom apathy.
The no observed effect concentration MOEC (96 h) Is 100 mg/l.
Based on the analytically determined concentrations the median lethal concentration LC 50 <96 h) is 100 < 220 mg/l.
The no observed effect concentration NOEC (96 h) is 100 mg/l.

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