Sodium diisobutylnaphthalenesulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sodium diisobutylnaphthalenesulphonate

EC Number:

248-326-4

EC Name:

Sodium diisobutylnaphthalenesulphonate

Cas Number:

27213-90-7

Molecular formula:

Not applicable (UVCB substance)

IUPAC Name:

sodium 2,3-bis(2-methylpropyl)naphthalene-1-sulfonate

Test material form:

solid: particulate/powder

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 - < 220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

The purpose of this study was to determine the acute toxicity of test item on the Zebra fish (Brachydanio rerio HAM. and BUCH.) using a static procedure. Zebra fish were exposed aver a 96-hour period to a range of 5 concentrations spaced by a factor of about 2.2: 46.4, 100. 215, 464 and 1000 mg/l.

An untreated control was included. The study procedure was based an the OECD guideline.

Results (on the basis of the nominal concentrations):

LC 50 after 96 hours

GREATER 100 (MG/L)(1% SIGNIFICANCE LEVEL)

SMALLER 220 (MG/L)(1% SIGNIFICANCE LEVEL)

Symptoms:

apathy

No observed effect concentration (NOEC): 100 mg/l

Maximum concentration tested causing no mortality: 100 mg/l

Minimum concentration tested causing 100% mortality: 215 mg/l

 

Results (on the basis of the measured concentrations):

Values after 96 hours

LC 50:

GREATER 100 <MG/L) ( 1% SIGNIFICANCE LEVEL)

SMALLER 220 <MG/L) ( 1% SIGMIFICANCE LEVEL)

Na observed effect concentration (NOEC): 100 mg/l

Maximum concentration tested causing no mortality: 100 mg/l

Minimum concentration tested causing 100% mortality: 216 mg/l

The analytically detected concentrations were initially in the range of 99.5% - 105.6% and after about 96 hours between 100.0% - 103.4% of the nominal concentrations

Applicant's summary and conclusion

Conclusions:

Based on the nominal concentrations the median lethal concentration LC 50 <96 h) is > 100 < 220 mg/l.
Mortality (10/10) occurred from the concentration 215 mg/l upward.
Another substance-related effect was the symptom apathy.
The no observed effect concentration MOEC (96 h) Is 100 mg/l.
Based on the analytically determined concentrations the median lethal concentration LC 50 <96 h) is 100 < 220 mg/l.
The no observed effect concentration NOEC (96 h) is 100 mg/l.