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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1971
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1971
Report date:
1971

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Litchfield J T& Wilcoxon F A. A simplified method of evaluating dose-effect experiments. J. Pharmacol. Exp. Ther. 96:99-113, 1949
Principles of method if other than guideline:
Litchfield J T& Wilcoxon F A. A simplified method of evaluating dose-effect experiments. J. Pharmacol. Exp. Ther. 96:99-113, 1949
GLP compliance:
no
Test type:
acute toxic class method

Test material

Constituent 1
Chemical structure
Reference substance name:
Naphthalenesulfonic acids, branched and linear Bu derivs., sodium salts
EC Number:
293-346-9
EC Name:
Naphthalenesulfonic acids, branched and linear Bu derivs., sodium salts
Cas Number:
91078-64-7
Molecular formula:
Not applicable (a generic molecular formula can not be provided for this specific UVCB substance)
IUPAC Name:
Naphthalenesulfonic acids, branched and linear Bu derivs., sodium salts
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
water

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
1 790 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 14 days
Sex:
female
Dose descriptor:
LD50
Effect level:
3 300 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 14 days

Any other information on results incl. tables

Oral administration

Administration was performed by means of a gavage.

Male rats

A.I. dose

mg/kg

Toxicological Results

after 14 days

Signs of

intoxication

Died

after no.

days

start

end

500

0/15

-

-

-

1000

0/15/15

2h

2d

-

1500

0/15/15

2h

2d

-

1750

4/15/15

2h

3d

1-3

1850

11/15/15

1.5h

2d

1

2000

12/15/15

Ih

3d

1-2

2250

15/15/15

Ih

-

1

 

Results: DL50 (14 days) 1790 mg A.I./kg (1700-1890)

S = 1.1

Highest dose without findings: 500 mg/kg

Lowest lethal dose: 1750 mg/kg

Female rats

A.I. dose

mg/kg

Toxicological Results

after 14 days

Signs of

intoxication

Died

after no.

days

start

end

500

0/0/15

-

-

-

1000

0/0/15

-

-

-

2500

2/15/15

40'

3d

2

3000

5/15/15

15'

5d

1-2

3500

10/15/15

10'

7d

1

4000

11/15/15

8'

7d

1

4500

14/15/15

6'

-

1

5000

15/15/15

4'

-

1

 

Result: DL50 (14 days) 3300 mg A.I./kg (3025-3600)

S = 1.2

Highest dose without findings: 1000 mg/kg

Lowest lethal dose: 2500 mg/kg

Female rabbits

A single oral dose of 1000 mg/kg to 3 rabbits respectively did not cause any distinct signs of intoxication.

Result: DL50 (14 days) > 1000 mg A.I./kg.

Female dogs

A single oral dose of 500 mg/kg to 2 dogs caused vomiting within one hour. However, no signs of intoxication could be determined.

Signs of intoxication

The signs of intoxication (deterioration of general health, labored breathing) occurred in mice and rats within 2 hours.

These signs could be observed from 2 to 7 days. Death occurred within 1 to 3 days.

Intraperitoneal injection

The active ingredient was injected into the abdominal cavity of the animals.

Male rats

A.I. dose

mg/kg

Toxicological Results

after 14 days

Signs of

intoxication

Died

after no.

days

start

end

10

0/0/15

-

-

-

25

0/15/15

1.5h

Id

—_

50

0/15/15

30'

4d

-

100

0/15/15

20'

5d

-

150

0/15/15

15'

6d

4

175

3/15/15

13'

7d

3-6

200

5/15/15

10'

7d

1-6

225

10/15/15

8'

7d

1-5

240

9/15/15

5'

7d

1-4

250

15/15/15

5'

-

-

 

Result: DL50 (14 days) 213 mg A.I./kg (200-226)

S = 1.1

Highest dose without findings: 10 mg/kg

Lowest lethal dose: 175 mg/kg

Male mice

A.I. dose

mg/kg

Toxicological Results

after 14 days

Signs of

intoxication

Died

after no.

days

start

end

10

O/ 0/15

-

-

-

25

0/15/15

30'

Id

-

50

0/15/15

22'

2d

-

100

1/15/15

10'

5d

4

150

8/15/15

5'

7d

2-5

200

12/15/15

4'

7d

1-6

250

14/15/15

3'

-

1-5

400

15/15/15

2'

-

1-2

 

Result: DL50 (14 days) 155.5 mg A.I. / kg (134-181)

S = 1.3

Highest dose without findings: 10 mg/kg

Lowest lethal dose: 100 mg/kg

Signs of intoxication:

Within mainly 30 minutes after the injection the animals showed signs of a deterioration of general health and labored breathing. In some cases the signs of intoxication could be observed for up to 7 days. Death occurred within 1 to 6 days.

Intravenous injectionin male mice

The active ingredient was injected into the caudal vein of mice at a rate of 1 ml/min in physiological NaCl solution.

 

A.I. dose

mg/kg

Toxicological Results

after 14 days

Signs of

intoxication

Died

after no.

days

start

end

25

O/ 0/15

-

-

-

50

0/15/15

30'

3d

-

75

1/15/15

25'

3d

2

85

6/15/15

17'

6d

2

100

12/15/15

13'

7d

2

150

15/15/15

5'

-

1-2

 

Result: DL50 (14 days) 89.1 mg A.I. / kg (84-95)

S =1.1

Highest dose without findings: 25 mg/kg

Lowest lethal dose: 75 mg/kg

The sings of intoxication were similar to those following intraperitoneal injection. Death occurred here within 2 days.

Subcutaneous application to male rats

A 500 mg/kg b.w. dose of the active ingredient was applied in 25% aqueous solution to the dorsal skin of 5 male rats shorn on the previous day. The compound was left on the skin for 7 days.

No signs of intoxication were observed in the rats treated in this manner.

Result: DL50 > 500 mg A.I. / kg.

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: OECD GHS
Conclusions:
The DL50 in male rats (14 days) 1790 mg A.I./ kg (1700-1890) and DL50 in female rats (14 days) 3300 mg A.I./ kg (3025-3600)