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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

Ethanol:
Pseudomonas putida. 16hr TT =6500mg/l
Paramaecium caudatum. 4hr LC50 = 5800mg/l
Uronema parduczi. 48hr TT=6120mg/l
Chilomonas paramecium 48hr TT=>10000mg/l
Entosiphon sulcatum. 72hr TT = 65mg/l
Tetrahymena pyriformis. 9 hr IC50 = 13100mg/l
Tetrahymena pyriformis. 48 hr EC50 = 11963mg/l
Isopropanol:
The 16 hour Toxicity Threshold concentration of 1050 mg/L for Pseudomonas putida (Bringmann & Kühn, 1980) is selected as the value to use for PNECstp calculations.

Key value for chemical safety assessment

Additional information

There are no data available for the reaction mass.

Ethanol:

A number of studies have been carried out to assess the toxicity of ethanol to micro-organism using multiple species of protozoa and bacteria in fresh water. These studies that are reliable indicate that the substance may be slightly toxic to micro-organisms. The weight of evidence suggested that the substance is generally of low toxicity. The 'standard' bacteria (P. putida) was around the middle of the toxicity results. Ciliated protozoa are acceptable as surrogates to predict the toxicity in a WWTP; Paramecium meet this criteria (but not Entosiphon). The Paramecium figure is therefore selected as the key parameter from which to predict the hazard to degradation of substances in waste water treatment plants.

Isopropanol:

The toxicity of 2-propanol toPseudomonas putidawas assessed in a published non-guideline study that predates GLP requirements for excotoxicity studies (Bringmann & Kühn 1980). The study used a static freshwater system. Cell culture was maintained on nutrient agar slants and examined for purity periodically. The inoculum was prepared by growing the microorganism on nutrient agar plates for 24 hours. The cells were washed off from the medium and resuspended in a nutrient medium. By determining the extinction of the monochromatic radiation at 436 nm for a 10 mm layer of the bacterial suspension, the final turbidity value of the bacterial suspension was adjusted, by means of sterile saline, such that it corresponded to the extinction value of a Formazin standard suspension TE/F/436 nm = 10.