Reaction mass of ethanol and propan-2-ol

Toxicity to terrestrial arthropods

Description of key information

Isopropanol:
LC50 = 3.2% IPA in diet, 1 day, male Drosophila melanogaster (25.1 g/L in diet)
LC50 = 3.4% alcohol, 1 day, female Drosophila melanogaster (26.7 g/L in diet)
LC50 = 1.5% alcohol, 2 day, male Drosophila melanogaster (11.8 g/L in diet)
LC50 = 1.7% alcohol, 2 day, female Drosophila melanogaster (13.4 g/L in diet)
(David & Bocquet, 1976)

Additional information

Isopropanol:

The toxicity of 2-propanol to 2 strains of small fruit flies,Drosophila melanogasterandDrosophila simulans, was assessed in a published non-guideline study that predates GLP requirements for ecotoxicity studies (David & Bocquet, 1976). This laboratory study used larvae grown at low larval density on an axenic, killed yeast medium. After emergence, adults were separated into groups of 10 females and 10 males and kept on nutrient medium for three days. After three days, each group of flies is transferred into an air-tight plastic vial containing 2 mL of a 3% sucrose solution supplemented with the tested concentration of 2-propanol (7 concentrations in a geometric progression of ratio 1.25). Mortality was determined after 1, and 2 days of treatment. No further details on results were reported. The results indicate that the male fruit fly is more susceptible to 2-propanol toxicity than the female fruit fly.

 

Fruit flies are exposed to ethanol from rotting fruits and other alcohols can also be produced by microorganisms which may be found inDrosophilafood (David & Bocquet, 1976). The metabolism of the alcohols encountered in food probably has an adaptive ecological significance.

 

The 2-day LC₅₀value for 2-propanol was 1.5 and 1.7% IPA in diet (11.8 and 13.4 g/L in diet) for male and femaleD. melanogaster, respectively (3-day LC₅₀not reported). The 3-day LC₅₀value for 2-proponal was 1.0 and 1.3% IPA in diet (7.9 and 10.2 mg/L in diet) for male and femaleD. simulans, respectively.