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EC number: 203-412-0 | CAS number: 106-58-1
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- Appearance / physical state / colour
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- Endpoint summary
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- Ecotoxicological Summary
- Aquatic toxicity
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- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1996-03-05 - 1996-04-10
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Qualifier:
- according to guideline
- Guideline:
- ISO 7827 (Evaluation in an Aqueous Medium of the "Ultimate" Aerobic Biodegradability of Organic Compounds - Method by Analysis of Dissolved Organic Carbon (DOC))
- Principles of method if other than guideline:
- GLP-guideline study; only one replicate with test substance was considered in the assessment of the degradation
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 1,4-Dimethylpiperazin
- Analytical purity: > 99%
- Lot/batch No.: Abl. Nr. 32-1659 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge taken from a laboratory WWTP fed with domestic and synthetic sewage.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 32 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: inorganic medium according to guideline
- Test temperature: ambient
- pH: no data
- pH adjusted: no
- Aeration of dilution water: no data
- Continuous darkness: yes
- Other:
TEST SYSTEM
- Culturing apparatus: Shaken flasks cultured under aerobic conditions and constant temperature
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: continuous aeration as prescribed by the guideline
- Measuring equipment: DOC analyser (Shimadzu TOC 500 and TOC 5000)
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes
- Other:
SAMPLING
- Sampling frequency: on days 0, 1, 3, 5, 7, 11, 14, 17, 21, 24, 27, 28
- Sample storage before analysis: no data
CONTROL AND BLANK SYSTEM
- Inoculum blank: BC, 2 replicates
- Abiotic sterile control: PC, 2 replicates
- Toxicity control: RS, 2 replicates
- Other: inhibition control (IH, 2 replicates); adsorption control (AC, 2 replicates)
STATISTICAL METHODS: not applicable - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 90
- Sampling time:
- 28 d
- Details on results:
- At test termination (28 days) the substance was degraded by ca. 90 % in replicate No. 1 (PS1), whereas in the second replicate (PS2) no degradation was observed after 28 days. In PS2 the first significant degradation was measured after 32 days (19%), followed by 88% and 92% after 35 and 36 days, respectively. Therefore, a second test using the activated sludge taken from PS1 was performed. In this test with adapted activated sludge a biodegradation of > 90 % within 6 days was recorded.
- Results with reference substance:
- Degradation of control substance:
94 % after 7 day(s) - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- With batch PS1, the 10-days window was met. At the end of the test, after 28 days, the test substance was degraded to 89%. The degradation of the reference substance aniline was 94 % after 7 days. Elimination of the test substance by abiotic processes or adsorption was less than 10 % after 28 and 7 days, respectively. The degradation in the inhibition control after 14 days was > 35%. Considering a longer test duration, results of PS1 and PS2 would have differed by < 20%.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-04-17 - 2008-05-15
- Reliability:
- 2 (reliable with restrictions)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 1,4-Dimethylpiperazin
- Analytical purity: 100%
- Lot/batch No.: 1901-2/2005 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- test item concentration: 100 mgL
- Reference item: aniline (100 mg/L)
- Activated sludge: 30 mg/L
- Temperature: 25±1 °C
- Duration: 28 days
TEST SYSTEM
- Number of culture flasks/concentration: 6 - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 5.6
- Sampling time:
- 28 d
- Results with reference substance:
- The reference substance was degraded at least 60% within 9 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In this DOC removal test conducted according to OECD guideline 301C, no biodegradation of the test substance was observed after 28 days. Test performance was demonstrated to be good (as shown by the results with the reference item and the toxicity control) and all validity criteria were met. The results of the test can be considered reliable. The test substance is not readily biodegradable.
Referenceopen allclose all
Degradation kinetics:
Day |
Elimination (%) |
|||||
Toxicity control |
Inhibition control |
Abiotic control |
Adsorption control |
Test substance |
||
PS1 |
PS2 |
|||||
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
4 |
-4 |
0 |
3 |
3 |
7 |
3 |
4 |
0 |
5 |
2 |
-1 |
-1 |
5 |
60 |
44 |
0 |
-5 |
-2 |
-3 |
7 |
94 |
43 |
2 |
-11 |
-3 |
-3 |
11 |
96 |
44 |
-2 |
|
-5 |
0 |
14 |
101 |
47 |
-1 * |
|
1 |
6 |
17 |
102 |
44 |
-7 |
|
-16 |
0 |
21 |
98 |
35 |
-6 |
|
9 |
-8 |
24 |
93 |
42 |
-4 |
|
90 |
-5 |
27 |
94 |
41 |
-7 |
|
90 |
-6 |
28 |
96 |
48 |
-10 |
|
89 |
-11 |
32 |
|
|
|
|
|
19 |
35 |
|
|
|
|
|
88 |
36 |
|
|
|
|
|
92 |
* 1 mL of HgCl2stock solution was added
Degradation results of the additional test (PS3) with adapted inoculum:
-8% after 1 day
98% after 6 days
93% after 7 days
Vessel n° | Sample description | BOD (mg) | |||
7th day | 14th day | 21st day | 28th day | ||
1 | 2 water + test item | 0.0 | 0.0 | 0.0 | 0.0 |
2 | sludge + test item | 2.1 | 3.5 | 4.9 | 5.5 |
3 | sludge + test item | 2.0 | 3.3 | 4.8 | 5.9 |
4 | sludge + test item | 2.3 | 3.3 | 4.8 | 5.6 |
5 | control blank | 3.7 | 6.3 | 7.6 | 7.9 |
6 | sludge + aniline | 63.7 | 75.4 | 77.6 | 78.3 |
Description of key information
The key study was performed equivalent to OECD guideline 301C under GLP conditions (Huntsman, 2008). Only 5.6 % degradation was observed with the test substance after 28 days (DOC removal). The results support the conclusion that the biodegradation of the test substance is slow. Therefore, the test substance is considered not readily biodegradable. Adaptation of the micro-organisms is an important process for the degradation rate of the test substance in the environment. Results from an OECD 301A study (BASF, 1996) indicate that the test substance shows enhanced biodegradability but under acclimated conditions.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
One study describing a ready biodegradability test was identified for the substance. The study of BASF (1996) describes a 28-days DOC die-away test and is performed according to OECD 301A. The study was assigned a Klimisch reliability score of 2 (reliable with restrictions) as only one replicate with the test substance was considered in the assessment of the biodegradation of the substance. The test substance was degraded by ca. 90 % in replicate No. 1 (PS1) and the 10-day window was met, whereas in the second replicate (PS2) no degradation was observed after 28 days. In PS2 the first significant degradation was measured after 32 days (19%), followed by 88% and 92% after 35 and 36 days, respectively. Therefore, a second test using the activated sludge taken from PS1 was performed. In this test with adapted activated sludge a biodegradation of > 90 % within 6 days was recorded. The reference substance (aniline) showed up to 94 % degradation after 7 days. Over 35 % of a control substance was degraded after 15 days in the presence of the test substance. Therefore, it can be concluded that the test results are valid. In nonadapted sludge from domestic sewage treatment plants, the degradation of the substance is very slow, with lagphases of more than 30 days, while in inoculum mixed with adapted sludge the lag phases are much shorter. The 28-day DOC die-away test with adapted sludge concluded that 1,4-dimethylpiperazine is readily biodegradable.
In another study, the test substance was tested according to MITI (I) (OECD 301C) (Huntsman, 2008). The study was assigned a klimisch score of 2. Activated sludge was used as inoculum (30 mg/L), pH 7. The test concentration was 100 mg/L, and the reference substance used was aniline. After 14 days, 3.3% of the test substance was biodegraded, compared to > 60% of the reference substance. After 28 days, 5.6% of the substance was degraded. However, the results support the conclusion that the biodegradation of the test substance is slow under non acclimated, aerobic conditions.
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