1,4-dimethylpiperazine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

(adopted on 1981, 12th may; inhalation hazard test)

Deviations:

yes

Remarks:

Animals were observed for only 7 days, no details only animal husbandry, exposure period of 8 hours, concentration of test substance in air mixture was not determined analytically

GLP compliance:

no

Test type:

other: inhalation hazard test (IHT)

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dimethylpiperazine

EC Number:

203-412-0

EC Name:

1,4-dimethylpiperazine

Cas Number:

106-58-1

Molecular formula:

C6H14N2

IUPAC Name:

1,4-dimethylpiperazine

Details on test material:

- Name of test material (as cited in study report): Jeffcat DMP

Specific details on test material used for the study:

- Name of test material (as cited in study report): N,N´-Dimethylpiperazin
- Analytical purity: 99 %

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 177 g (mean)

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

air

Details on inhalation exposure:

The test demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at the temperature chosen for vapour generation (20 °C). 3 rats (respec. 6 rats in the 8h experiment) per sex were exposed sequentially to the vapours, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted glassdisc in a glass cylinder for 1 h, 3 h or 8 h. The documentation of clinical signs was performed over a period of 8 days.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

>= 1 - <= 8 h

Remarks on duration:

1 h, 3 h or 8 h exposure

Concentrations:

1 h and 3 h exposure: 17.2 mg/L (mean); no data concerning 8 h exposure.
(nominal concentrations not verified by analytics).

No. of animals per sex per dose:

1 h and 3 h exposure: 6 animals
8 h exposure: 12 animals

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

other: Inhalation Risk Test

Based on:

test mat.

Remarks on result:

other: exposure of 1, 3 or 8 hours (3 h exposure: 1 rat died within 24 hours; 8 h exposure: 1 animal died within the exposure, 9 animals within 24 h and 1 animal within 72 h after exposure)

Mortality:

1 h exposure: no mortality observed.
3 h exposure: 1 rat died within 24 hours.
8 h exposure: 1 animal died within the exposure, 9 animals within 24 h and 1 animal within 72 h after exposure.

Clinical signs:

other: Rats showed escape attempts and signs of severe irritation of the mucous membranes. Bloody snout, nose and eye crusts. Lateral position and tumbling in the 8 h exposure group.

Body weight:

The surviving animals gained weight.

Gross pathology:

1 h exposure: no abnormalities observed.
3 h exposure: 1 animal showed ruffled fur and slight bloody crusts at the muzzle and orbital cavity. 1 animal had pulmonary emphysema with bronchitis.
8 h exposure: animals showed ruffled fur and bloody crust formation at the nose (4x), muzzles and orbital cavity (1x). 2 animals had pulmonary emphysema.

Any other information on results incl. tables

Mortality:

	24 h	7 days
1 h exposure	0/6	0/6
3 h exposure	1/6	1/6
8 h exposure	10/12	11/12

The inhalation of a enriched/saturated vapor-air-mixture caused mortality within 3 h. There is indication that the test substance causes local irritation to exposed tissue including respiratory tract.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

The inhalation of a enriched/saturated vapor-air-mixture caused mortality within 3 h. There is indication that the test substance causes local irritation to exposed tissue including respiratory tract.