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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1968
Report date:
1968

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Standardised study to determine the acute toxicity of the test item in mice after single i.p. application.
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-dimethylpiperazine
EC Number:
203-412-0
EC Name:
1,4-dimethylpiperazine
Cas Number:
106-58-1
Molecular formula:
C6H14N2
IUPAC Name:
1,4-dimethylpiperazine
Details on test material:
- Name of test material (as cited in study report): Jeffcat DMP
Specific details on test material used for the study:
- Name of test material (as cited in study report): N,N´-Dimethylpiperazin
- Analytical purity: 99 %

Test animals

Species:
mouse
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 19-36 g
No further data.

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Details on exposure:
No data
Doses:
200, 400, 640, 800 cm³/kg bw (170, 340, 544, 680 mg/kg bw - conversation in mg/kg is based on the density: d= 0.85 g/cm³)
The doses were administered as aqueous solutions of 8% (800 cm³/kg), 4% (400, 640 cm³/kg) and 2% (200 cm³/kg) test substance.
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Frequency of weighing: before the start of the study
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 544 - < 680 mg/kg bw
Based on:
test mat.
Remarks on result:
other: Corresponds to 640 and 800 cm³/kg bw; the mg/kg was calculated on the density d= 0.85 g/cm³. 680 mg/kg: all females and 4 males died within 24 hours post application; 544 mg/kg bw: one male and one female animal died within 24 hours post application.
Mortality:
680 mg/kg bw : all female animals and 4 males died within 24 hours post application
544 mg/kg bw : 1 male and 1 female animal died within 24 hours post application
340 mg/kg bw: one female animal died within 7 days post application
No mortalities in the 170 mg/kg bw dose group.
Clinical signs:
Immediately after application, dyspnoea, tremor, indicated morphine tails, shrunken flanks, high stepping gait, struggles in the 680 mg/kg bw dose group were observed. On the next day, the surviving animals showed clotted eyes, scrubby fur, accelerated respiration. 5 to 6 days after exposure, no clinical signs were observed.
Body weight:
no data
Gross pathology:
Animals that died: livers were full of blood, intestine and intraperitoneum were reddened
Animals sacrificed at the end of observation period: connation of liver and stomach in 10 animals

Applicant's summary and conclusion

Conclusions:
After intraperitoneal administration of the substance to mice, the LD50 was observed to be within the range of 544 and 680 mg/kg bw.