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EC number: 232-304-6 | CAS number: 8002-26-4 A complex combination of tall oil rosin and fatty acids derived from acidulation of crude tall oil soap and including that which is further refined. Contains at least 10% rosin.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998-05-25 to 1998-05-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study without restrictions. The study was conducted according to the appropriate OECD test guideline, and in compliance with GLP.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (Control), 625, 2500 and 10000 mg/L
- Sampling method: Samples of the test media were taken at the start and end of the test.
- Sample storage conditions before analysis: not reported. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was a poorly soluble complex mixture and therefore the test media were water-accommodated fractions (WAF) prepared by stirring for 23 hours with a vortex depth not exceeding 10% of the liquid depth. The media were then allowed to stand for 1 hour before drawing off the WAFs for testing.
WAFs were prepared at nominal loading rates of 625, 1250, 2500, 5000 and 10000 mg/L. The WAFs were renewed after each successive 24 hour exposure period.
- Controls: Dilution water - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: SAG 86.81
- Source (laboratory, culture collection): Laboratory stock culture maintained on a sterile sloped agar tube (OECD-Medium, 0.8% Agar), transferred to new agar tube every 2 months.
- Age of inoculum (at test initiation): ca. 72 hours
- Method of cultivation: Incubation on a shaker in 250 ml Erlenmeyer flasks, seterile, at 25ºC, 100-130 rpm, 10000 lux (Gro-Lux-Aquarstar) continuous illumination. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- not reported
- Test temperature:
- 25+/-2.0 ºC
- pH:
- 6.3 – 10.3 (The deviation exceeded the guideline maximum of 1.5 units but this was a consequence of the high levels of algal growth (algal cell density increased by a factor of 116 in 72 hours in the Controls) achieved in the test.
- Dissolved oxygen:
- not applicable
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal loading rates (first series): 75, 150, 300, 625 and 1250 mg/L
Nominal loading rates (second series): 0 (Control), 625, 1250, 2500, 5000 and 10000 mg/L.
Concentrations of total organic carbon were measured in the Control and 625, 2500 and 10000 mg/L WAFs at the start and end of the test. The corresponding measured concentrations were 0.9, 8.5, 16.1 and 34.1 mg/L at the start of the test and 1.6, 7.5, 16.9 and 34.0 mg/L at the end of the test (Table 3). The results indicate that the exposure concentrations were stable over the course of the test.
The test results are reported with reference to nominal loading rates. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks.
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): static test
- Initial cells density: 10000 cells/mL
- Control end cells density: 1160000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD 201 prepared in demineralised water
- Culture medium different from test medium: no
- Intervals of water quality measurement: Start and end of test
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 1000 lux (Gro-Lux Aquastar) continuous
EFFECT PARAMETERS MEASURED: cell densities after 24, 48 and 72 hours
- Determination of cell concentrations: counting chambers (Fuchs-Rosenthal: chamber factor 80000; Neubauer: chamber factor 4000000)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- ca. 3 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- water-accommodated fraction loading rate
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- ca. 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- water-accommodated fraction loading rate
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- ca. 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- water-accommodated fraction loading rate
- Basis for effect:
- other: growth rate and biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none reported
- Any stimulation of growth found in any treatment: no
- Effect concentrations exceeding solubility of substance in test medium: The tested loading rates exceeded the solubility of the test substance - Reported statistics and error estimates:
- The EL50 values were estimated from plots of percentage inhibion of biomass or growth rate against log concentration. The NOELR was estimated directly from the raw data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 72 h EL50 of circa 3300 mg/L and NOELR of circa 300 mg/L have been determined for the effects of the test substance on growth rate of Scenedesmus subspicatus. A 72 h EL50 of circa 2000 mg/L has been determined for effects on biomass in the same test.
Reference
Table 1. Test results - first series of test media - 72 hours
Nominal loading rate (mg/L) | Mean cell concentration (cells/mL) | Average specific growth rate | Inhibition of average specific growth rate (%) | Area under the growth curve | Inhibition of area under the growth curve (%) |
Control | 1160000 | 0.07 | - | 24652000 | - |
625 | 709333 | 0.06 | 10 | 17020000 | 31 |
1250 |
909333 | 0.06 | 5.1 | 21260000 | 14 |
2500 | 264000 | 0.05 | 31 | 6508000 | 74 |
5000 | 34833 | 0.02 | 74 | 906000 | 96 |
10000 | 163000 | 0.04 | 41 | 3508000 | 86 |
Table 2. Test results - second series of test media - 72 hours
Nominal loading rate (mg/L) | Inhibition of area under the growth curve (%) |
Control | - |
75 | 0 |
150 |
16 |
300 | 3.4 |
625 | 27 |
1250 | 29 |
Table 3. Results of TOC analysis of test media
Time (h) | Control | 625 mg/L | 2500 mg/L | 10000 mg/L |
0 | 0.9 | 8.5 | 16.1 | 34.1 |
72 | 1.6 | 7.5 | 16.9 | 34.0 |
Description of key information
The results for toxicity to algae and aquatic plants are summarised in Table 7.4. A 72h EL50 of approximately 3300 mg/L and NOELR of approximately 300 mg/L have been determined for the effect of DTO on growth rate of Scenedesmus subspicatus. These values are the lowest of those obtained in three reliable tests conducted on different samples of DTO.
Key value for chemical safety assessment
Additional information
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