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EC number: 232-304-6
CAS number: 8002-26-4
A complex combination of tall oil rosin and fatty acids derived from acidulation of crude tall oil soap and including that which is further refined. Contains at least 10% rosin.
2: BOD removal from the test flasks during the 28 day exposure
BOD Test substance
BOD Reference article
removal = BODtestsubstance
193.8 mg O2/L or 1.896 mg O2/mg test substance.
Ready Biodegradation: 73.2% in 28 days (OECD 301F)
biodegradation value of 73.2% (measured
as percentage of theoretical oxygen demand) was achieved in 28 days
using a method consistent with OECD Guideline 301 F (manometric
respirometer) for Distilled Tall Oil (DTO).
Although, information about the 10-day window was not available, the
degradation rate is very rapid for a very poorly soluble substance. The
reference substance attained 92% degradation by Day 14 and percentage
degradation between replicate test samples was ± 20%, satisfying the
validity criteria of the test.
supporting studies, DTO was readily biodegradable in a GLP test
conducted according to OECD 301D (Closed Bottle Test) using an equal
volume mixture of three commercially available products. The substance
achieved significant levels of biodegradation (60% in 28 days) but
failed to meet the 10-day window. The biodegradability of a commercial
grade of DTO was measured in a GLP test according to OECD Guideline 301E
(Modified OECD Screening Test). The test medium comprised an aqueous
extract of the product, prepared by loading culture medium with 10 g/l
test substance and stirring for 23 hours, standing for 1 hours and
removing the aqueous extract by siphoning. The aqueous extract attained
64% degradation in 28 days.
assessment of biodegradability of the test substance is based on the
test conducted in accordance to OECD 301F because this is more
applicable to a poorly soluble substance like DTO. As
a whole, it can be concluded that DTO is readily biodegradable for
purposes of classification and labelling.
biodegradation of the individual constituents of the substance are
Table 4.3 shows the summary from BIOWIN
Ultimate model for the constituent blocks of DTO, and the resulting
biodegradation rating used for modelling degradation in the waste water
treatment plant. The ratings are:
Biodegradable meeting the ’10-day window’
biodegradable not meeting the ’10-day window’
biodegradable fulfilling criteria
biodegradable not fulfilling criteria
NB Not biodegradable.
However the predicted biodegradation rating
for the resin acids constituents in Block 4 – 6 were overwritten based
on available reliable measured ready biodegradation data for these
groups of constituents. A ready biodegradation of 64% in 28 days using
OECD 301B has been determined for the resin acid constituents of DTO (Schaefer
EC and Haberlein D (1998)).
4.3: Biodegradation class for the constituents of DTO
Low boiling fatty acids
C16 saturated - C18.3 unsaturated fatty acids
C18 saturated - C24 saturated and unsaturated fatty acids
Tetracosanol and dehydrated sterol
Sitosterols and analoque
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