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The long-term effects of MBTS on mortality, biomass development, morphological changes and reproduction to the earthworm Eisenia fetida in 5% artificial soil were tested in the laboratory according to OECD Guideline 222. At concentrations up to and including 1000 mg test item/kg soil dry weight the test item had no adverse effects on either mortality, biomass development, morphological changes and reproduction to the test species (ECT 2017). The derived NOEC is considered to be >= 1000 mg test item / kg soil dw and the derived LOEC is considered to be >1000

mg test item / kg soil dw for either mortality, biomass and reproduction as basis of effect. Conclusively, MBTS shows no toxic chronic effects on terrestrial invertebrates.

 

The long-term effects of di(benzothiazol-2-yl) disulphide on terrestrial plant seedling emergence and seedling growth was investigated according to the OECD guideline 208. The test species were

two monocotyledonous species, Avena sativa (oat) and Allium cepa (onion), as well as four dicotyledonous species, Brassica napus (rape), Cucumis sativus (cucumber), Pisum sativum (pea) and Solanum lycopersicum (tomato). Duration of the test was either 14 days (A. cepa, A. sativa, P. sativum), or 15 days (C. sativus, S. lycopersicum), or 16 days (B. napus). No effects were observed with regard to emergence, survival and growth as basis of effect. Thus, the test substance showed a NOEC of >= 1000 mg/kg dw and a LOEC of >1000 mg/kg dw for all test species (ECT 2017). Therefore, it can be concluded that di(benzothiazol-2-yl) disulphide has no chronic effects to terrestrial plants.

 

The effects on soil micro-organisms of the substance di(benzothiazol-2 -yl) disulphide were investigated according to the OECD guideline 216. The test item had no adverse effects on N-transformation in lucerne amended soil during 28 days at test item concentrations of up to and including 1000 mg/kg soil dry weight. Due to the lacking of adverse effects effective concentrations (ECX) could not be calculated,

Consequently, ECX are considered to be higher than 1000 mg/kg soil dry weight which was the highest tested concentration. Accordingly, LOAEC and NOAEC are > and ≥ 1000 mg/kg soil dry weight, respectively (ECT 2017). In conclusion, di(benzothiazol-2 -yl) disulphide is not considered to have adverse effects on soil micro-organisms.

In summary, di(benzothiazol-2 -yl) disulphide shows no terrestrial toxicity.

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