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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
not specified
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Reference substance:
aniline
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Results with reference substance:
62 % degradation of aniline within 28 days
Interpretation of results:
not readily biodegradable
Conclusions:
For 2,2'-disulfanediylbis(1,3-benzothiazole) 0 % degradation within 28 days was observed and is hence classified as not readily biodegradable.
Executive summary:

For 2,2'-disulfanediylbis(1,3-benzothiazole) 0 % degradation within 28 days was observed. The test was conducted following the OECD-Guideline 301 C-modified MITI Test. 2,2'-disulfanediylbis(1,3-benzothiazole) is hence classified as not readily biodegradable (Bayer AG, 1988).

Description of key information

For 2,2'-disulfanediylbis(1,3-benzothiazole) 0 % degradation within 28 days was observed. The test was conducted following the OECD-Guideline 301 C-modified MITI Test. 2,2'-disulfanediylbis(1,3-benzothiazole) is hence classified as not readily biodegradable (Bayer AG, 1988). In the EU-risk assessment for CBS (CAS: 95-33-0), information regarding the biodegradability of the potential metabolites of MBTS, such as MBT, BT, BTon, and MeSBT, is also summarized and discussed. The described metabolites are also not readily biodegradable. Only when the inoculi were adapted under special conditions, certain level of degradation of MBT, BT, BTon, and MeSBT was observed. Such processes are expected in biological treatment plant, but not in the environment.

Key value for chemical safety assessment

Additional information

No respiration inhibition on activated sludge is expected, because the test concentration (i.e. 100 mg/l) of MBTS is much lower than the effective concentrations, such as an 3h-EC50 >10000 mg/l for MBTS.