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EC number: 609-530-2
CAS number: 38172-91-7
The test item 2-Propyn-1-ol, compd. with methyloxirane was
assessed in the micronucleus assay for its potential to induce
micronuclei in polychromatic erythrocytes (PCE) in the bone marrow of
The test item was dissolved in sterile water, which was also used
as vehicle control. The volume administered orally was 10 mL/kg b.w.. 24
h and 48 h after a single administration of the test item the bone
marrow cells were collected for micronuclei analysis.
At least five males per test group were evaluated for the
occurrence of micronuclei. Per animal 2000 polychromatic erythrocytes
(PCEs) were scored for micronuclei.
To investigate a cytotoxic effect due to the treatment with the
test item the ratio between polychromatic and normochromatic
erythrocytes was determined in the same sample and reported as the
number of PCEs per 2000 erythrocytes.
on results of pre-experiments doses of 250, 500 and 1000 mg/kg
b.w. were selected for the main experiment. However, due to high
mortality observed at 1000 mg/kg (4 of 7 males of the 48 hours group)
this dose was not appropriate for evaluation in accordance with the
guidelines (less than 5 animals available for evaluation in the 48 hour
group and maximum tolerated dose level clearly exceeded). Additional
groups of male mice were treated including vehicle and positive control
in a second main experiment in order to fulfil the OECD guideline
requirements for a valid study. Finally, following dose levels of the
test item were investigated and evaluated :
24 h preparation interval: 125b), 250a), and
48 h preparation interval: 500b)mg/kg b.w.
main experimentb)Second main experiment
In the second main experiment one of 7 males male died after
treatment with the high dose (500 mg/kg b.w., 48 hours post-treatment).
After treatment with the test item the number of PCEs was not
substantially decreased as compared to the mean value of PCEs of the
vehicle control thus indicating that 2-Propyn-1-ol, compd. with
methyloxirane did not exert a cytotoxic effect in the bone marrow.
In comparison to the corresponding vehicle controls there was no
statistically significant or biologically relevant enhancement in the
frequency of the detected micronuclei at any preparation interval and
dose level after administration of the test item. The mean values of
micronuclei observed after treatment with 2-Propyn-1-ol, compd. with
methyloxirane were below or near to the value of the vehicle control
groups. Only the micronucleus frequency found in the high dose group
(500 mg/kg, 48 hours treatment) was statistically significantly higher
(0.108%) compared to the concurrent vehicle control value (0.030%) which
was incidentally quite low. However, the micronucleus frequency in the
500 mg/kg (48h) group as well as in all other test item treated dose
groups at any preparation interval were very well within the
laboratory’s historical vehicle control data (0.010-0.250%, mean
0.108%). Thus, the observed statistical significance at 500 mg/kg (48h)
was not considered to have any biological relevance.
40 mg/kg b.w. cyclophosphamide administered orally was used as
positive control which showed a statistically significant increase of
induced micronucleus frequency.
In conclusion, it can be stated that during the study described
and under the experimental conditions reported, the test item 2-Propyn-1-ol,
compd. with methyloxirane did not induce micronuclei as determined
by the micronucleus test in the bone marrow cells of the mouse.
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