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EC number: 203-004-2
CAS number: 102-08-9
The objective of this study was to evaluate the potential irritant
properties of the test item for the eye, 1,3-Diphenyl-2 -thiourea,
following a single administration to rabbits.
The study was performed according to the international guidelines
(OECD guideline No. 405, 24th April 2002, and Commission Regulation (EC)
No. 440/2008, B.5, 30 May 2008) and in compliance with the principles of
Good Laboratory Practice.
The test item, 1,3-Diphenyl-2-thiourea, was first administered to
a single female New Zealand White rabbit.
As mean value from grading at 24, 48 and 72 hours after
instillation was < 2 for conjunctival edema (chemosis) and for
conjunctival redness, < 1 for iris lesion and for corneal opacity, the
test item was administered in the left eye of two other animals.
The test item was administered inthe conjunctival sac of the left eye.
The right eye remained untreated and served as control.
A quantity of 0.1 g/animal was used.
The eyes of the first animal were not rinsedjust after the 1-hour
scoring, whereas, both eyes of two other animals were rinsed with a
sterile isotonic saline solution (0.9% NaCl).
Each animal was observed once a day for mortality and clinical
signs.Ocular reactions were observed approximately 1 hour, 24, 48 and
72 hours after the administration and then daily until the reversibility
of the ocular reactions. The mean values of the scores for chemosis,
redness of the conjunctiva, iris lesions and corneal opacity were
calculated for each animal. Body weight was recorded on the day of
treatment and the end of the observation period.
On completion of the observation period, the animals were sacrificed
then discarded without macroscopic post-mortem examination.
A slight or moderate chemosis of the conjunctiva was observed in all
animals on day 1, then a slight chemosis persisted until day 5 in 1/3
A slight or moderate redness of the conjunctiva was noted in all animals
on day 1, and it persisted on day 2 in 1/3 animals and until day 4 in
Mean scores calculated for each animal over 24, 48 and 72 hours were as
. chemosis: 1.0, 0.0 and 0.0; showing no significant eye
. redness of the conjunctiva: 1.3, 0.3 and 0.0; showing no
significant eye irritation,
. iris lesions: 0.0, 0.0 and 0.0; showing no significant eye
. corneal opacity: 0.0, 0.0 and 0.0; showing no significant eye
In conclusion, the test item is not classified as irritating to
the eyes according to the criteria of CLP Regulation.
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