[No public or meaningful name is available]

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30-03-2016 -29-04-2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

no

Remarks:

study was performed without GLP, reasons: unknown

Test material

Test material information

Constituent 1

Reference substance name:

Recombinant eADF4(C48-CS25C) protein from E. Coli (K12 HMS174(DE3)-plasmid pAMS029) fermentation and subsequent purification

Molecular formula:

Not applicable (UVCB substance)

IUPAC Name:

Recombinant eADF4(C48-CS25C) protein from E. Coli (K12 HMS174(DE3)-plasmid pAMS029) fermentation and subsequent purification

Test material form:

solid: bulk

Details on test material:

Test Item:
eADF4(C48-CS25C)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

Supernatant of the homogenizate of activated sludge from the Taunusstein-Bleidenstadt wastewater treatment plant, batch 30.03.2016

Duration of test (contact time):

29 d

Initial test substance concentration

Initial conc.:

39.1 mg/L

Based on:

ThCO2

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (CO2 evolution)

Value:

85

Sampling time:

29 d

% Degradation 2

Key result

Parameter:

% degradation (CO2 evolution)

Value:

83

Sampling time:

28 d

% Degradation 3

Key result

Parameter:

% degradation (CO2 evolution)

Value:

71

Sampling time:

20 d

% Degradation 4

Key result

Parameter:

% degradation (CO2 evolution)

Value:

43

Sampling time:

8 d

% Degradation 5

Key result

Parameter:

% degradation (CO2 evolution)

Value:

1

Sampling time:

4 d

BOD5 / COD results

Results with reference substance:

Converted to 84 % CO2 after 28 days; the threshold value for the the validity criterion of >60 % degradation within 10 days was already exceeded within 8 days (66 %).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test substance is readily biodegradable following OECD 301B.

Executive summary:

The average degree of degradation of the test item was 80% after 28 days. Accordingly, the test substance is to be defined as "readily biodegradable". The degree of degradation in the first test solution was 76%, in the second test solution a degradation rate of 85% was determined.

The included toxicity control showed no indication of an inhibitory effect. Therefore, when applying the 10-day-window, the test substance is readily biodegradable following OECD 301B.