Bis(N,N',N''-trimethyl-1,4,7-triazacyclononane)-trioxo-dimanganese (IV) di(hexafluorophosphate)monohydrate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: no data on GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

not specified

Principles of method if other than guideline:

no data

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Bis(N,N',N''-trimethyl-1,4,7-triazacyclononane)-trioxo-dimanganese (IV) di(hexafluorophosphate)monohydrate

EC Number:

411-760-1

EC Name:

Bis(N,N',N''-trimethyl-1,4,7-triazacyclononane)-trioxo-dimanganese (IV) di(hexafluorophosphate)monohydrate

Cas Number:

116633-53-5

Molecular formula:

C18 H42 Mn2 N6 O3 . 2 F6 P

IUPAC Name:

dimanganese(4+) bis(1,4,7-trimethyl-1,4,7-triazonane) bis(hexafluoro-λ⁵-phosphanuide) hydrate trioxidandiide

Details on test material:

no data

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

no data

Test solutions

Vehicle:

not specified

Details on test solutions:

no data

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

no data

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

no data

Test conditions

Hardness:

162 mg/l as CaCO3

Test temperature:

no data

pH:

no data

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

no data

Details on test conditions:

The test was conducted under static renewal conditions. Daphnia were exposed to a concentration of 100 mg/l. Analysis of the test substance indicated that exposure concentration remained stable throughout the test.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

There was 28% mortality at 48 hours. These Daphnia appeared to have died as a result of being caught in the surface film rather than as a direct response to the inherent toxicity of the test substance.

Results with reference substance (positive control):

no data

Reported statistics and error estimates:

no data

Any other information on results incl. tables

Values in mg/L
	24hr	48hr
EC50	>100	>100

% LOSS IN CONCENTRATION OVER TEST PERIOD The loss of concentration was measured as 0% during the 48 hour renewal period.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

EC50 at 48hr >100 mg/l

Executive summary:

There was 15% immobilisation at 24 hours and 28% at 48 hours. The notifier considers that this was due to extended periods of time in the surface layer which led to stress and was not due to toxicity.