Methanesulfonamide, N-[2-[(4,6-dimethoxy-1,3,5-triazin-2-yl)carbonyl]-6-fluorophenyl]-1,1-difluoro-N-methyl-

Biodegradation in soil

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in soil: simulation testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 307 (Aerobic and Anaerobic Transformation in Soil)

Version / remarks:

2002

Qualifier:

according to guideline

Guideline:

other: Commission Directive 95/36/EC amending Council Directive 91/414/EEC, 1995

Qualifier:

according to guideline

Guideline:

other: US EPA OCSPP Fate, Transport and Transformation Test Guideline No. 835.4100 / 835.4200, 2008

Qualifier:

according to guideline

Guideline:

other: Japanese MAFF New Test Guidelines 12 Nousan 8147 Annex No. 2-5-2, 2001

GLP compliance:

yes (incl. QA statement)

Remarks:

Ministerium für Arbeit, Gesundheit und Soziales des Landes Nordrhein-Westfalen

Test type:

laboratory

Radiolabelling:

yes

Oxygen conditions:

aerobic

Soil classification:

USDA (US Department of Agriculture)

Soil no.:

#1

Soil type:

sandy loam

% Clay:

7

% Silt:

18

% Sand:

75

% Org. C:

2.2

pH:

6.7

CEC:

9.8 meq/100 g soil d.w.

Bulk density (g/cm³):

1.25

Soil no.:

#2

Soil type:

clay loam

% Clay:

31

% Silt:

38

% Sand:

31

% Org. C:

5

pH:

7.4

CEC:

20.6 meq/100 g soil d.w.

Bulk density (g/cm³):

1

Soil no.:

#3

Soil type:

other: Loamy, mixed, mesic Typic Argudalfs

% Clay:

17

% Silt:

30

% Sand:

53

% Org. C:

3.4

pH:

5.4

CEC:

10.8 meq/100 g soil d.w.

Bulk density (g/cm³):

1.15

Soil no.:

#4

Soil type:

other: Loamy, mixed, mesic Typic Argudalfs

% Clay:

17

% Silt:

56

% Sand:

27

% Org. C:

2.5

pH:

6.8

CEC:

14.3 meq/100 g soil d.w.

Bulk density (g/cm³):

1.11

Details on soil characteristics:

SOIL COLLECTION AND STORAGE
- The soils were freshly sampled from the field and sieved to ≤ 2 mm
- Geographic location:
#1: Laacher Hof AXXa, Monheim, Germany (GPS coordinates: N 51° 04.6' E 006° 53.5')
#2: Dollendorf II, Blankenheim, Germany (GPS coordinates: N 50° 22.9' E 006° 43.0')
#3: Laacher Hof Wurmwiese, Monheim, Germany (GPS coordinates: N 51° 04.9' E 006° 55.3')
#4: Hoefchen am Hohenseh 4a, Burscheid, Germany (GPS coordinates: N 51° 04.0' E 007° 06.3')

Soil No.:

#1

Duration:

120 d

Soil No.:

#2

Duration:

120 d

Soil No.:

#3

Duration:

120 d

Soil No.:

#4

Duration:

120 d

Soil No.:

#1

Initial conc.:

0.267 mg/kg soil d.w.

Based on:

act. ingr.

Soil No.:

#2

Initial conc.:

0.267 mg/kg soil d.w.

Based on:

act. ingr.

Soil No.:

#3

Initial conc.:

0.267 mg/kg soil d.w.

Based on:

act. ingr.

Soil No.:

#4

Initial conc.:

0.267 mg/kg soil d.w.

Based on:

act. ingr.

Parameter followed for biodegradation estimation:

radiochem. meas.

Soil No.:

#1

Temp.:

20.3°C

Humidity:

55%

Microbial biomass:

781 mg microbial carbon per kg of soil dry weight

Soil No.:

#2

Temp.:

20.3°C

Humidity:

55%

Microbial biomass:

3660 mg microbial carbon per kg of soil dry weight

Soil No.:

#3

Temp.:

20.3°C

Humidity:

55%

Microbial biomass:

705 mg microbial carbon per kg of soil dry weight

Soil No.:

#4

Temp.:

20.3°C

Humidity:

55%

Microbial biomass:

1166 mg microbial carbon per kg of soil dry weight

Details on experimental conditions:

EXPERIMENTAL DESIGN
- The test systems consisted of 300 mL Erlenmeyer flasks each containing 100 g soil (dry weight equivalents)
- The test vessels were equipped with traps for the collection of carbon dioxide and volatile organic compounds but permeable for oxygen.
- Soil moisture was adjusted to 55 ± 5%
- For acclimation, the test systems were placed in the temperature controlled climatic chamber for 5 days prior to application
- Oxygen conditions: Aerobic
- Light conditions: Dark
- Temperature: mean 20.3 °C (20.0 – 20.9 °C)
- Stock solution: 14C-labeled test item was dissolved in 25 mL acetonitrile resulting in a stock solution containing 4.35 MBq/mL
- The stock solution was stored frozen protected from light
- The test item was applied dropwise onto the soil surface in 390 μl methanol / water 1/1 (v/v).
- Duplicate samples were analyzed 0, 0.08 (2 hours), 0.25 (6 hours), 1, 2, 3, 7, 14, 21, 41, 59, 90 and 120 days after treatment.
- At each sampling interval, the soil was extracted three times by shaking at ambient temperature and once by microwave-accelerated extraction at 70 °C using acetonitrile / water 4/1 (v/v).

Soil No.:

#1

% Recovery:

100

Remarks on result:

other: Total recovery range 95.8 - 104%

Soil No.:

#2

% Recovery:

100

Remarks on result:

other: Total recovery range 95.9 - 105.0%

Soil No.:

#3

% Recovery:

100

Remarks on result:

other: Total recovery range 95.3 - 103.7%

Soil No.:

#4

% Recovery:

100

Remarks on result:

other: Total recovery range 96.1 - 103.1%

Soil No.:

#1

Parameter:

radiochem. meas.

Remarks:

< LOD within three to six weeks

Remarks on result:

not determinable

Soil No.:

#2

Parameter:

radiochem. meas.

Remarks:

< LOD within three to six weeks

Remarks on result:

not determinable

Soil No.:

#3

Parameter:

radiochem. meas.

Remarks:

< LOD within three to six weeks

Remarks on result:

not determinable

Soil No.:

#4

Parameter:

radiochem. meas.

Remarks:

< LOD within three to six weeks

Remarks on result:

not determinable

Soil No.:

#1

DT50:

1.1 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#2

DT50:

0.7 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#3

DT50:

1.1 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#4

DT50:

0.9 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#1

DT50:

0.5 d

Type:

other: Double first order in parallel

Temp.:

20.3 °C

Remarks on result:

other: DT90 = 1.8 days

Soil No.:

#2

DT50:

0.3 d

Type:

other: Double first order in parallel

Temp.:

20.3 °C

Remarks on result:

other: DT90 = 1.0 days

Soil No.:

#3

DT50:

0.5 d

Type:

other: Double first order in parallel

Temp.:

20.3 °C

Remarks on result:

other: DT90 = 1.9 days

Soil No.:

#4

DT50:

0.4 d

Type:

other: Double first order in parallel

Temp.:

20.3 °C

Remarks on result:

other: DT90 = 1.4 days

Transformation products:

yes

No.:

#1

No.:

#2

No.:

#3

No.:

#4

No.:

#5

No.:

#6

Details on transformation products:

Six degradation products ≥ 10% were identified (see above). Small amounts of extractable and charaterisable by chromatography residues were observed with no one component exceeding 6.0% at any sampling interval.

Volatile metabolites:

no

Residues:

no

The route and rate of degradation of the test item was studied in four soils under aerobic conditions in the dark in the laboratory for 120 days at 20.3 °C and 55% of the maximum water holding capacity. A study application rate of nominal 267 μg per kg soil dry weight was applied. The results show rapid degradation of the test item in soil under aerobic conditions. The DT50 value in soil ranges (recalculated to 12°C) from 0.6 to 1.1 days. Six degradation products ≥ 10% AR were detected and identified as AE 1887196-dihydro, AE 1887196-oxoacetamide, AE 1887196-O-desmethyl, AE 1887196-di-Odesmethyl, AE 1887196-dihydro-O-desmethyl and AE 1887196-dihydro-di-O-desmethyl. Formation of significant amounts of carbon dioxide demonstrates complete mineralization of the test item.

Reference 2

Endpoint:

biodegradation in soil: simulation testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 307 (Aerobic and Anaerobic Transformation in Soil)

Version / remarks:

2002

Qualifier:

according to guideline

Guideline:

other: Commission Directive 95/36/EC amending Council Directive 91/414/EEC, 1995

Qualifier:

according to guideline

Guideline:

other: US EPA OCSPP Fate, Transport and Transformation Test Guideline No. 835.4100 / 835.4200, 2008

Qualifier:

according to guideline

Guideline:

other: Japanese MAFF New Test Guidelines 12 Nousan 8147 Annex No. 2-5-2, 2001

GLP compliance:

yes (incl. QA statement)

Remarks:

Ministerium für Arbeit, Gesundheit und Soziales des Landes Nordrhein-Westfalen

Test type:

laboratory

Radiolabelling:

yes

Oxygen conditions:

aerobic

Soil classification:

USDA (US Department of Agriculture)

Soil no.:

#1

Soil type:

sandy loam

% Clay:

7

% Silt:

18

% Sand:

75

% Org. C:

2.2

pH:

6.7

CEC:

9.8 meq/100 g soil d.w.

Bulk density (g/cm³):

1.25

Soil no.:

#2

Soil type:

clay loam

% Clay:

31

% Silt:

38

% Sand:

31

% Org. C:

5

pH:

7.4

CEC:

20.6 meq/100 g soil d.w.

Bulk density (g/cm³):

1

Soil no.:

#3

Soil type:

other: Loamy, mixed, mesic Typic Argudalfs

% Clay:

17

% Silt:

30

% Sand:

53

% Org. C:

3.4

pH:

5.4

CEC:

10.8 meq/100 g soil d.w.

Bulk density (g/cm³):

1.15

Soil no.:

#4

Soil type:

other: Loamy, mixed, mesic Typic Argudalfs

% Clay:

17

% Silt:

56

% Sand:

27

% Org. C:

2.5

pH:

6.8

CEC:

14.3 meq/100 g soil d.w.

Bulk density (g/cm³):

1.11

Details on soil characteristics:

SOIL COLLECTION AND STORAGE
- The soils were freshly sampled from the field and sieved to ≤ 2 mm
- Geographic location:
#1: Laacher Hof AXXa, Monheim, Germany (GPS coordinates: N 51° 04.6' E 006° 53.5')
#2: Dollendorf II, Blankenheim, Germany (GPS coordinates: N 50° 22.9' E 006° 43.0')
#3: Laacher Hof Wurmwiese, Monheim, Germany (GPS coordinates: N 51° 04.9' E 006° 55.3')
#4: Hoefchen am Hohenseh 4a, Burscheid, Germany (GPS coordinates: N 51° 04.0' E 007° 06.3')

Soil No.:

#1

Duration:

126 d

Soil No.:

#2

Duration:

126 d

Soil No.:

#3

Duration:

126 d

Soil No.:

#4

Duration:

126 d

Soil No.:

#1

Initial conc.:

0.267 mg/kg soil d.w.

Based on:

act. ingr.

Soil No.:

#2

Initial conc.:

0.267 mg/kg soil d.w.

Based on:

act. ingr.

Soil No.:

#3

Initial conc.:

0.267 mg/kg soil d.w.

Based on:

act. ingr.

Soil No.:

#4

Initial conc.:

0.267 mg/kg soil d.w.

Based on:

act. ingr.

Parameter followed for biodegradation estimation:

radiochem. meas.

Soil No.:

#1

Temp.:

20.3°C

Humidity:

5.7-57.2%

Microbial biomass:

690 mg microbial carbon per kg of soil dry weight

Soil No.:

#2

Temp.:

20.3°C

Humidity:

51.6-55.3%

Microbial biomass:

3231 mg microbial carbon per kg of soil dry weight

Soil No.:

#3

Temp.:

20.3°C

Humidity:

50.4-57.2%

Microbial biomass:

690 mg microbial carbon per kg of soil dry weight

Soil No.:

#4

Temp.:

20.3°C

Humidity:

51.3-55.3%

Microbial biomass:

1066 mg microbial carbon per kg of soil dry weight

Details on experimental conditions:

EXPERIMENTAL DESIGN
- The test systems consisted of 300 mL Erlenmeyer flasks each containing 100 g soil (dry weight equivalents)
- The test vessels were equipped with traps for the collection of carbon dioxide and volatile organic compounds but permeable for oxygen.
- Soil moisture was adjusted to 55 ± 5%
- Oxygen conditions: Aerobic
- Light conditions: Dark
- Temperature: mean 20 ± 2 °C (mean: 20.1 °C, min: 19.8 °C, max. 20.6 °C)
- Stock solution: 14C-labeled test item was dissolved in 8.79 mL acetonitrile resulting in a stock solution of 1 mg/mL (3.79 MBq/mL) labeled test item
- The stock solution was stored frozen protected from light
- The test item was applied dropwise onto the soil surface in 390 μl methanol / water 1/1 (v/v).
- Duplicate samples were processed and analyzed after 0, 0.08, 0.25, 1, 2, 3, 6, 13, 21, 41, 64, 91 and 126 days of incubation.
- At each sampling interval, the soil was extracted three times by shaking at ambient temperature and once by microwave-accelerated extraction at 70 °C using acetonitrile / water 4/1 (v/v).

Soil No.:

#1

% Recovery:

100

Remarks on result:

other: Total recovery range 95.6 - 103%

Soil No.:

#2

% Recovery:

100

Remarks on result:

other: Total recovery range 99.4 - 99.3%

Soil No.:

#3

% Recovery:

100

Remarks on result:

other: Total recovery range 95.3 - 101.6%

Soil No.:

#4

% Recovery:

100

Remarks on result:

other: Total recovery range 96.8 - 102.3%

Soil No.:

#1

Parameter:

radiochem. meas.

Remarks:

< LOD within three to six weeks

Remarks on result:

not determinable

Soil No.:

#2

Parameter:

radiochem. meas.

Remarks:

< LOD within three to six weeks

Remarks on result:

not determinable

Soil No.:

#3

Parameter:

radiochem. meas.

Remarks:

< LOD within three to six weeks

Remarks on result:

not determinable

Soil No.:

#4

Parameter:

radiochem. meas.

Remarks:

< LOD within three to six weeks

Remarks on result:

not determinable

Soil No.:

#1

DT50:

1.1 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#2

DT50:

0.6 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#3

DT50:

1.3 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#4

DT50:

1.1 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#1

DT50:

0.5 d

Type:

other: Double first order in parallel

Temp.:

20.1 °C

Remarks on result:

other: DT90 = 2.0 days

Soil No.:

#2

DT50:

0.3 d

Type:

other: Double first order in parallel

Temp.:

20.1 °C

Remarks on result:

other: DT90 = 1.2 days

Soil No.:

#3

DT50:

0.6 d

Type:

other: Double first order in parallel

Temp.:

20.1 °C

Remarks on result:

other: DT90 = 2.1 days

Soil No.:

#4

DT50:

0.5 d

Type:

other: Double first order in parallel

Temp.:

20.1 °C

Remarks on result:

other: DT90 = 1.7 days

Transformation products:

yes

No.:

#1

No.:

#2

No.:

#3

No.:

#4

No.:

#5

No.:

#6

Details on transformation products:

Six degradation products ≥ 10% were identified (see above). Small amounts of extractable and charaterisable by chromatography residues were observed with no one component exceeding 4.7% at any sampling interval.

Volatile metabolites:

no

Residues:

no

The route and rate of degradation of the test item was studied in four soils under aerobic conditions in the dark in the laboratory for 126 days at 20.1 °C and 55% of the maximum water holding capacity. A study application rate of nominal 267 μg per kg soil dry weight was applied. The results show rapid degradation of triafamone in soil under aerobic conditions. The DT50 value in soil ranges (recalculated to 12°C) from 0.6 to 1.3 days. Six degradation products ≥ 10% AR were detected and identified as AE 1887196-dihydro, AE 1887196-dihydro-O-desmethyl, AE 1887196-dihydro-di-O-desmethyl, AE 1887196-O-desmethyl, AE 1887196-di-O-desmethyl and AE 1887196-oxoacetamide. Formation of significant amounts of carbon dioxide demonstrates complete mineralization of the test item.

Reference 3

Endpoint:

biodegradation in soil: simulation testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 307 (Aerobic and Anaerobic Transformation in Soil)

Qualifier:

according to guideline

Guideline:

other: US EPA OCSPP Fate, Transport and Transformation Test Guideline No. 835.4100 / 835.4200

Qualifier:

according to guideline

Guideline:

other: Japanese MAFF New Test Guidelines Annex No. 2-5-3

GLP compliance:

yes (incl. QA statement)

Remarks:

Ministerium für Arbeit, Gesundheit und Soziales des Landes Nordrhein-Westfalen

Test type:

laboratory

Radiolabelling:

yes

Oxygen conditions:

aerobic/anaerobic

Soil classification:

USDA (US Department of Agriculture)

Soil no.:

#1

Soil type:

silt loam

% Clay:

17

% Silt:

60

% Sand:

23

% Org. C:

1

pH:

6.4

CEC:

12.1 meq/100 g soil d.w.

Bulk density (g/cm³):

1.16

Soil no.:

#2

Soil type:

loam

% Clay:

17

% Silt:

32

% Sand:

51

% Org. C:

1.7

pH:

5.6

CEC:

9.9 meq/100 g soil d.w.

Bulk density (g/cm³):

1.1

Soil no.:

#3

Soil type:

clay loam

% Clay:

31

% Silt:

42

% Sand:

27

% Org. C:

4.9

pH:

7.3

CEC:

20.5 meq/100 g soil d.w.

Bulk density (g/cm³):

0.97

Details on soil characteristics:

SOIL COLLECTION AND STORAGE
- The soils were freshly sampled from the field (upper horizon of 0 to 20 cm) and sieved to ≤ 2 mm
- Geographic location:
#1: Hoefchen am Hohenseh 4a, Burscheid, Germany (GPS coordinates: N 51° 04.0' E 007° 06.3')
#2: Laacher Hof Wurmwiese, Monheim, Germany (GPS coordinates: N 51° 04.9' E 006° 55.3')
#3: Dollendorf II, Blankenheim, Germany (GPS coordinates: N 50° 22.9' E 006° 43.0')

Soil No.:

#1

Duration:

120 d

Soil No.:

#2

Duration:

122 d

Soil No.:

#3

Duration:

122 d

Soil No.:

#1

Initial conc.:

0.4 mg/kg soil d.w.

Based on:

act. ingr.

Soil No.:

#2

Initial conc.:

0.4 mg/kg soil d.w.

Based on:

act. ingr.

Soil No.:

#3

Initial conc.:

0.4 mg/kg soil d.w.

Based on:

act. ingr.

Parameter followed for biodegradation estimation:

radiochem. meas.

Soil No.:

#1

Temp.:

20.1°C

Microbial biomass:

1137 mg microbial carbon per kg of soil dry weight

Soil No.:

#2

Temp.:

20.1°C

Microbial biomass:

213-354 mg microbial carbon per kg of soil dry weight

Soil No.:

#3

Temp.:

20.1°C

Microbial biomass:

479-1007 mg microbial carbon per kg of soil dry weight

Details on experimental conditions:

EXPERIMENTAL DESIGN
- The test systems consisted of 300 mL Erlenmeyer flasks each containing 100 g soil (dry weight equivalents)
- The test vessels were placed in a nitrogen-flooded box
- Soil moisture: Soil was flooded with 140 mL oxygen-free ultrapure water
- Oxygen conditions: Anaerobic (oxygen-free atmosphere in the closed test systems)
- Light conditions: Dark
- Temperature: mean 20 ± 2 °C (mean: 20.1 °C, min: 19.8 °C, max. 20.4 °C)
- Stock solution: 14C-labeled test item was dissolved in 4.39 mL acetonitrile by sonication resulting in a stock solution of 1 mg/mL (3.79 MBq/mL) labeled test item
- The stock solution was stored frozen in the dark
- The test item was applied dropwise onto the soil surface using an adjustable pipette.
- Duplicate samples were processed and analyzed after 0, 1, 2, 3, 8, 14, 30, 59, 91 and 120 days (Soil #1) and 0, 1, 2, 3, 7, 14, 29, 60, 92 and 122 days (Soil #2, #3).
- At each sampling interval, the soil was extracted three times at ambient temperature and once by microwave-accelerated extraction at 70 °C using acetonitrile / water 4/1 (v/v).

Soil No.:

#1

% Recovery:

100

Remarks on result:

other: Total recovery range 97.1 - 104.2%

Soil No.:

#2

% Recovery:

100

Remarks on result:

other: Total recovery range 97.0 - 101.5%

Soil No.:

#3

% Recovery:

100

Remarks on result:

other: Total recovery range 91.9 - 101.5%

Soil No.:

#1

Parameter:

radiochem. meas.

Remarks:

< LOD at the end of the test duration

Remarks on result:

not determinable

Soil No.:

#2

Parameter:

radiochem. meas.

Remarks:

not detectable at the end of the test duration

Remarks on result:

not determinable

Soil No.:

#3

Parameter:

radiochem. meas.

Remarks:

not detectable at the end of the test duration

Remarks on result:

not determinable

Soil No.:

#1

DT50:

1.3 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#2

DT50:

3.9 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#3

DT50:

1.3 d

Type:

other: Double first order in parallel

Temp.:

12 °C

Remarks on result:

other: recalculated half-life to 12°C with Arrhenius equation

Soil No.:

#1

DT50:

0.6 d

Type:

other: Double first order in parallel

Temp.:

20.1 °C

Remarks on result:

other: DT90 = 7.3 days (Chi2 Error 1.4)

Soil No.:

#2

DT50:

1.8 d

Type:

other: Double first order in parallel

Temp.:

20.1 °C

Remarks on result:

other: DT90 = 13.5 days (Chi2 Error 1.0)

Soil No.:

#3

DT50:

0.6 d

Type:

other: Double first order in parallel

Temp.:

20.1 °C

Remarks on result:

other: DT90 = 5.7 days (Chi2 Error 1.1)

Transformation products:

yes

No.:

#1

No.:

#2

No.:

#3

No.:

#4

Details on transformation products:

Four transformation products > 10% AR were identified (see above). The unidentified radioactivity (max. 8.5%) could be distributed into up to five components with no single component exceeding 3.1%.

Volatile metabolites:

no

Residues:

no

The route and rate of degradation of the test item was studied in three soils under flooded anaerobic conditions in the dark in the laboratory. Following a short aerobic incubation phase in the dark, samples were incubated for a maximum of 122 days at 20.1 °C under anaerobic conditions. The results show rapid degradation of triafamone in soil under aerobic and anaerobic conditions. The DT50 value in soil for the anaerobic incubation phase ranges (recalculation to 12°C) from 1.3 to 3.8 days. Four degradation products ≥ 10% were detected and identified as AE 1887196-dihydro, AE 1887196-O-desmethyl, AE 1887196-dihydro-O-desmethyl and AE 1887196-oxazolidine-dione, of which AE 1887196- oxazolidine-dione was formed in the anaerobic incubation phase. The main degradation product AE 1887196-dihydro is steadily degraded under anaerobic conditions.

Description of key information

DT50 = 0.6 - 3.9 days (OECD 307, aerobic and anaerobic conditions, recalculated to 12 °C)

In soil the substance will be degraded within a short time period.

Additional information

The route and rate of degradation of triafamone (CAS No. 874195-61-6) was studied in four soils under aerobic conditions and in three soils under flooded anaerobic conditions. The studies conducted according to OECD guideline 307 and GLP in the dark at approximately 20.1 - 20.3 °C. A study application rate of nominal 267μg per kg soil dry weight was applied in the aerobic tests and of 400μg per kg soil dry weight in the anaerobic test. The results show that triafamone degrades fast in soil under aerobic and anaerobic conditions. Under aerobic conditions the DT50 value ranges (recalculated to 12 °C) from 0.6 to 1.3 days and under anaerobic conditions the DT50 value ranges (recalculated to 12° C) from 1.3 to 3.9 days. Under aerobic conditions six main degradation products were detected and identified as AE 1887196-dihydro, AE 1887196-oxoacetamide, AE 1887196-O-desmethyl, AE 1887196-di-Odesmethyl, AE 1887196-dihydro-O-desmethyl and AE 1887196-dihydro-di-O-desmethyl. One further degradation product was identified as AE 1887196-oxazolidine-dione, which was formed in the anaerobic incubation phase.