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REACH

Toluene-4-sulphonohydrazide

EC number: 216-407-3 | CAS number: 1576-35-8

Life Cycle description
Uses advised against

Environmental fate & pathways

Hydrolysis

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September-November 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

GLP-study carried according to guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Version / remarks:

Adopted 13th April, 2004

Deviations:

GLP compliance:

yes (incl. QA statement)

Radiolabelling:

Analytical monitoring:

yes

Buffers:

The sterilized buffer solutions of different pH values (pH 4.00, 7.00 and 9.00) were prepared from the annex (Buffer mixtures of Clark and Lubs) of the OECD test guideline. This buffer solutions were aerated to free from nitrogen for 5 minutes before preparation of the test solution. The pH of each buffer solution was check with a calibrated pH-meter before exposure test substance.

Details on test conditions:

Group description
(1) Tier 1
Test concentration : 0.01 M (1,875.995 mg/L) for pH 4.00, 7.00, 9.00
Replicate : Duplicate per test sample (pH 4.00, 7.00 and 9.00)
Temperature : 50.0±0.5oC
Test duration : 5 days
Test solution : 200 mL per replicate
(2) Tier 2 and Tier 3
Test concentration : 0.01 M (1,875.995 mg/L) for pH 4.00, 7.00, 9.00
Replicate : Duplicate per test sample (pH 4.00, 7.00 and 9.00)
Temperature : 20.0±0.5 oC, 50.0±0.5 oC, 60.0±0.5 oC
Test duration : 90 % hydrolysis or 30 days (performed until whichever comes first)
Test solution : 200 mL per replicate
2) Exposure method
(1) All glassware, reagent-grade water and buffer solutions used in the test were sterilized. A series of procedures related to the test were performed on clean bench to avoid the biodegradation.
(2) A dark glass container was used to prevent photolytic effect during the test period, a water bath was covered with a cover to avoid the light.
3) Sterility confirmation test
Sterility confirmation test was performed to confirm that the test solution for each concentration was aseptic. But, if no degradation (<10 %) was observed, the sterility test was not performed.

Duration:

30 d

pH:

Temp.:

20 °C

Initial conc. measured:

0.01 mol/L

Remarks:

Test duration either 30 days or until 90% hydrolyis obtained

Duration:

30 d

pH:

Temp.:

50 °C

Initial conc. measured:

0.01 mol/L

Remarks:

Duration 30 days or until 90% hydrolysis obtained

Duration:

30 d

pH:

Temp.:

60 °C

Initial conc. measured:

0.01 mol/L

Remarks:

Duration 30 days or until 90% hydrolysis obtained

Duration:

30 d

pH:

Temp.:

20 °C

Initial conc. measured:

0.01 mol/L

Remarks:

Duration 30 days or until 90% hydrolysis obtained

Duration:

30 d

pH:

Temp.:

50 °C

Initial conc. measured:

0.01 mol/L

Remarks:

Duration 30 days or until 90% hydrolysis obtained

Duration:

30 d

pH:

Temp.:

60 °C

Initial conc. measured:

0.01 mol/L

Remarks:

Duration 30 days or until 90% hydrolysis obtained

Duration:

30 d

pH:

Temp.:

20 °C

Initial conc. measured:

0.01 mol/L

Remarks:

Duration 30 days or until 90% hydrolysis obtained

Duration:

30 d

pH:

Temp.:

50 °C

Initial conc. measured:

0.01 mol/L

Remarks:

Duration 30 days or until 90% hydrolysis obtained

Duration:

30 d

pH:

Temp.:

60 °C

Initial conc. measured:

0.01 mol/L

Remarks:

Duration 30 days or until 90% hydrolysis obtained

Number of replicates:

Positive controls:

Negative controls:

yes

Statistical methods:

No information

Transformation products:

yes

No.:

Details on hydrolysis and appearance of transformation product(s):

Hydrazine was actually not detected, but on the basis of read-across to OBSH (80-51-3) and due to chemical structure considerations, it is obvious that hydrazine is also formed.

pH:

Temp.:

20 °C

Hydrolysis rate constant:

0.005 h-1

DT50:

138.63 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Confidence interval: 0.005-0.006

pH:

Temp.:

50 °C

Hydrolysis rate constant:

0.019 h-1

DT50:

36.48 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Confidence interval: 0.018-0.020

pH:

Temp.:

60 °C

Hydrolysis rate constant:

0.056 h-1

DT50:

12.38 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Confidence interval: 0.049-0.063

Key result

pH:

Temp.:

20 °C

Hydrolysis rate constant:

0.018 h-1

DT50:

38.51 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Confidence interval: 0.015-0.021

pH:

Temp.:

50 °C

Hydrolysis rate constant:

0.368 h-1

DT50:

1.88 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Confidence interval: 0.321-0.416

pH:

Temp.:

60 °C

Hydrolysis rate constant:

0.824 h-1

DT50:

0.84 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Confidence interval: 0.653-0.995

pH:

Temp.:

20 °C

Hydrolysis rate constant:

0.017 h-1

DT50:

40.77 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Confidence interval: 0.016-0.019

pH:

Temp.:

50 °C

Hydrolysis rate constant:

0.384 h-1

DT50:

1.81 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Confidence interval: 0.310-0.458

pH:

Temp.:

60 °C

Hydrolysis rate constant:

0.854 h-1

DT50:

0.81 h

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: Confidence interval: 0.751-0.958

Validity criteria fulfilled:

yes

Conclusions:

The below give the measured 1. order hydrolysis rate constant (krate) and 1. order hydrolysis halflife (DT50) at various pHs and temperatures.
pH T (°C) krate (hr-1) DT50 (hr)
4 20 0.005 [0.005-0.006] 139
7 20 0.018 [0.015-0.021] 39
9 20 0.017 [0.016-0.019] 41
4 50 0.019 [0.018-0.020] 36
7 50 0.368 [0.321-0.416] 1.9
9 50 0.384 [0.310-0.458] 1.8
4 60 0.056 [0.049-0.063] 12
7 60 0.824 [0.653-0.995] 0.84
9 60 0.854 [0.751-0.958] 0.81

Executive summary:

The study was performed to assess abiotic hydrolytic transformations of the CELLCOM-H in the aquatic environment at pH 4∼9 and temperatures 20^oC, 50^oC, 60^oC according to OECD 111. The hydrolysis rate constants and the half-life according to the exposure conditions was as follows.

pH	T (°C )	krate (hr-1)	DT50 (hr)
4	20	0.005 [0.005-0.006]	139
7	20	0.018 [0.015-0.021]	39
9	20	0.017 [0.016-0.019]	41
4	50	0.019 [0.018-0.020]	36
7	50	0.368 [0.321-0.416]	1.9
9	50	0.384 [0.310-0.458]	1.8
4	60	0.056 [0.049-0.063]	12
7	60	0.824 [0.653-0.995]	0.84
9	60	0.854 [0.751-0.958]	0.81

Description of key information

The hydrolysis of TSH was tested according to OECD Guideline no. 111 at three temperatures (20 °C, 50 °C, 60 °C) and 3 pHs (4, 7, 9).

The results are given in below table, where krate is the overall 1. order rate constant.

pH	T (°C )	krate (hr-1)	DT50 (hr)
4	20	0.005 [0.005-0.006]	139
7	20	0.018 [0.015-0.021]	39
9	20	0.017 [0.016-0.019]	41
4	50	0.019 [0.018-0.020]	36
7	50	0.368 [0.321-0.416]	1.9
9	50	0.384 [0.310-0.458]	1.8
4	60	0.056 [0.049-0.063]	12
7	60	0.824 [0.653-0.995]	0.84
9	60	0.854 [0.751-0.958]	0.81

Key value for chemical safety assessment

Half-life for hydrolysis:: 38.51 h
at the temperature of:: 20 °C

Additional information

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