Reaction mass of Phenol, dodecyl-, sulfurized, calcium salts and benzene and butene and calcium carbonate and calcium dihydroxide and sulphur trioxide

Short-term toxicity to aquatic invertebrates

Description of key information

Key study:  EL50 of EC 903-161-3 was concluded to be greather than 100 mg/L (the highest value tested ) and this value was adjusted to account for the base oil solvent content of the sample tested (40.2%) , therefore a EL50 value of > 59.8 mg/L is the key value derived.
Supporting study: EL50 of an appropriate analogue substance was concluded to be greater than 1000 mg/L (the highest loading rate tested).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

59.8 mg/L

Additional information

The acute toxicity of EC-903-161-3 to Daphnia magna was assessed by conducting a limit test with test solutions prepared at a loading rate of 100 mg EC-903-161-3/L. The test was conducted over a 48 h period under static conditions, in accordance with OECD (2004) Guideline 202, following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances.

Based on the results of a range finding test, a limit test was conducted with test solution prepared as Water Accommodated Fraction (WAF) at nominal loading rate of 100 mg EC-903-161-3/L. A control of untreated Daphnia medium was also included.

No immobile D. magna were noted throughout the test period.

The 48 h EL50 of EC-903-161-3 to D. magna was concluded to be greater than 100 mg/L and the no observed effect loading

rate (NOELR) was concluded to be 100 mg/L.

A supporting study- The study was performed to determine the acute toxicity of the water accommodated fraction (WAF) of an appropriate analouge test substance to the daphnid, Daphnia magna, in a static system for a 48-hour period. The loading rates for this test were 1000mg/L, 400mg/L, 160mg/L, 64.0mg/L, and 25.6mg/L. Laboratory dilution water (BW3) was used as a control. Four replicates at each loading rate were tested. Each chamber was completely filled with the appropriate WAF, leaving no headspace. Samples were removed from each

WAF and the control on Day 0 and at termination (composite of replicates). Samples were analyzed for Total Organic Carbon (TOC).

The results of the TOC analysis indicated that the concentration of total organic carbon in the WAFs was below the minimum quantitation limit of 1 ppm. The 48- hour 50% Effect Loading rate (EL50) value was > l000 mg/L based on exposure to the WAF of the test substance. The information in this study is considered to be useful in the support of the data for the Registration material.

From the results of both studies it is clear that both the Registration substance and the analogue substance do not show signs of aquatic toxicity in the short term test of Daphnia. Results were adjusted for the presence of oil.