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EC number: 220-479-1
CAS number: 2781-00-2
The potential of the test item
1,3(4)-bis(tert-butylperoxyisopropyl)benzene to induce mutations
at the TK (Thymidine Kinase) locus in L5178Y mouse lymphoma cells was
evaluated according to the
international guidelines (OECD 476, Commission Directive No. B17) and in
compliance with the Principles of Good Laboratory Practice.
After a preliminary toxicity test,
1,3(4)-bis(tert-butylperoxyisopropyl)benzene was tested in
two independent experiments, with and without a metabolic activation
system, the S9 mix, prepared from a liver microsomal fraction (S9
fraction) of rats induced with Aroclor 1254.
The dose-levels for the positive
controls were as follows:
. without S9 mix: methylmethane
sulfonate (MMS), used at a final concentration of 25 µg/mL, (3-hour
treatment) or 5 µg/mL (24-hour treatment),
Experiments without S9 mix
Using a treatment volume of 50 µL/20
mL, the selected dose-levels were as follows:
. 0.063, 0.125, 0.25, 0.5, 1 and 2 mM
for the first experiment (3-hour treatment),
At the end of the 3-hour treatment, a
slight precipitate was noted in the culture medium at 2 mM.
Following the 3-hour treatment, no
noteworthy toxicity was observed at any of the tested dose-levels.
Experiments with S9 mix
In the first experiment, a slight
precipitate was observed in the culture medium at 2 mM at the end of the
In the first experiment, a 28-50%
decrease in the Adj. RTG was noted at dose-levels ≥ 0.25 mM.
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