Thiophene, tetrahydro-, 1,1-dioxide, 3-(C9-11-isoalkyloxy) derivs., C10-rich

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Sept 12, 1979 to January 18, 1980

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study report was conclusive, done to a valid guideline

Qualifier:

according to guideline

Guideline:

other: "Law Concerning the Examination and Regulation of Manufacture, etc., of Chemical Substance" (Japan. 1973. Law No. 117)

Deviations:

not specified

GLP compliance:

no

Remarks:

Study predates GLP regulations.

Radiolabelling:

no

Details on sampling:

2 weeks, 4 weeks, 6 weeks and 8 weeks (mean exposure concentrations and concentrations of compound in fish)

Details on preparation of test solutions, spiked fish food or sediment:

Dispersion of test compound for Acute Toxicity Test and Bioaccumulation Test:
Dispersing agent : HCO-40 (Polyoxyethylene Hydrogenated Castor Oil)
Method of dispersion:
Test chemical (2.2 g) and HCO-40 (22 g) were dissolved in 300 ml of acetone. Acetone was then removed by evaporation. The mixture was dispersed in dechlorinated tap water and filled up to 1 L.

Test concentrations:
Determination of exposure levels of test compound:
(1) The concentration which can be determined with accuracy is approximately 5 ppm (see Figures 4 and 5 attached)
(2) Concentration up to centuple (200 times) has been found possible at the preparation for test media analysis.
(3) In anticipation of recovery of 87% and the possible 30% loss of the concentration of the test material in the aquarium.
((5/(200 x (87 x 100) x ((100-30)/100)) = 0.05 ppm
As to the higher concentration level, it was fixed at decuple (10 times) of the lower level.

Nominal test concentrations (ppm) were as follows:
Compound S-296 HCO-40
Level 1 0.5 5
Level 2 0.05 0.5
Actual test concentrations are given in attached Table 1.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Test Fish for Acute Toxicity Test (TLm) : Adult orange-red killifish (Oryzias latipes)
average weight 0.3 g
(passed mercuric chloride test*)
* refer to Kenji Tabata "The proposal for standard TLm test using orange-red killifish as test fish. Journal of Water and Waste, 14, 1297 - 1303 (1972)

Test fish for the Bioaccumulation test:
Carp (Cyprinus carpio)
Average weight 26.7 g
Average length 10.2 cm
Lipid content* 3.4%

*E G. Bligh and W.J.Dyer, Can. J. Biochem.Physiol., 37, 911 (1956)

External disinfection and acclimation
(1) External disinfection
Bathed in the 10 ppm chlorotetracycline hydrochloride solution for 24 hours
(2) Acclimation : 14 days at 25 + or - 1ºC

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Hardness:

no data

Test temperature:

25 ± 1ºC for Acute Toxicity Test and Bioaccumulation test

pH:

no data

Dissolved oxygen:

no data

TOC:

no data

Salinity:

no data

Details on test conditions:

Test condition for Bioaccumulation Test
(a) Aquatron (Continuous flow)
Vessel : 100 litre glass aquarium
Flow Rate: 576 l/d
Flow ratio: Stock solution : diluent = 4 ml/min : 400 ml/min

Nominal and measured concentrations:

0.5 ppm Level 1
0.05 ppm Level 2

Reference substance (positive control):

not specified

Type:

BCF

Value:

31 dimensionless

Basis:

whole body w.w.

Time of plateau:

8 wk

Calculation basis:

steady state

Remarks on result:

other: mean value for both level 1 and level 2 exposure groups

Remarks:

Conc.in environment / dose:0.5

Details on results:

48 hr TLm value : 7.2 ppm (w/v) (see Figure 3 attached) for Acute Toxicity Test

Bioaccumulation test results:
Appearance of Fish : normal
Concentration factor: The calculated results are given in Table 2 (under results incl. tables section).

Explanation for the expression of the test result:
The concentration which can be determined with accuracy is approximately 5 ppm (see Figures 4 and 5 attached)

when fish body weight is 30 g
final liquid quantity is 5 ml
recovery is 83%
dilution factor is 2

this corresponds to approximately

((5/((30/(2x5)) x (83/100)) = 2.01 ppm the concentration in the fish body, and is divide this by the mean aquarium concentration of 8 weeks, it corresponds to 5.3 times of the first level and 54 times of the second level.

As the concentration of compound in the testing vessel was found to be less than the nominal concentration, following additional experiment was carried out.

The test condition and results are as follows.

Test condition
Vessel : 100 litre glass aquarium
Flow rate of test water : 576 l/day
Test temperature: 25 ± 1°C
Test period : 2 days
Nominal test concentration : 0.05 ppm(w/v)

Actual Concentration without fish 0.052 ppm
Actual Concentration with 10 fish 0.040 ppm

It seems that the presence of fish in the test vessel lowers the concentration of test compound. However, as shown in Table 1(under results incl. tables section), approximately constant exposure concentration was maintained. From this point of view, there is no problem in the credibility of test results.

Table 1: Mean Exposure Concentration (ppm)

	2W	4W	6W	8W
Level 1	0.39	0.38	0.39	0.38
Level 2	0.036	0.034	0.036	0.037

Table 2: Calculated Concentration factor

		2W	4W	6W	8W
Level 1	Measurement 1	24	42	41	28
Level 1	Measurement 2	46	27	28	34
Mean					31
Level 2	Measurement 1	23	33	38	29
Level 2	Measurement 2	21	21	34	32
Mean					31

Validity criteria fulfilled:

yes

Conclusions:

The presence of fish in the test vessel lowers the concentration of test compound. The BCF after 8 weeks exposure has a mean value of 31 using a test concentration of 0.5 ppm and 0.05ppm.

Executive summary:

Method

In a bioaccumulation study conducted in accordance with the 'Law Concerning the Examination and Regulation of Manufacture, etc., of Chemical Substance (Japan. 1973. Law No. 117), the bioaccumulation of the substance was determined (predated GLP guidelines).

Result

The presence of fish in the test vessel lowers the concentration of test compound. The BCF after 8 weeks exposure has a mean value of 31 using a test concentration of 0.5 and 0.05 ppm.

Description of key information

The BCF of the substance was determined to be 31 after eight weeks exposure; study conducted in accordance with 'Law Concerning the Examination and Regulation of Manufacture, etc., of Chemical Substance' (Japan. 1973. Law No. 117); Anonymous (1980)

Key value for chemical safety assessment

BCF (aquatic species):

31 dimensionless

Additional information

In a bioaccumulation study conducted in accordance with the 'Law Concerning the Examination and Regulation of Manufacture, etc., of Chemical Substance (Japan. 1973. Law No. 117), the bioaccumulation of the substance was determined (predated GLP guidelines). Under the conditions of the test, the BCF of the substance was determined to be 31 after eight week’s exposure.