2-methylbutan-1-ol

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: scientifically acceptable study (ip application) For justification of read-across please refer to section 13.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Principles of method if other than guideline:

Internal BASF method was used

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Pentan-1-ol

EC Number:

200-752-1

EC Name:

Pentan-1-ol

Cas Number:

71-41-0

Molecular formula:

C5H12O

IUPAC Name:

pentan-1-ol

Details on test material:

- Name of test material (as cited in study report): pentanol-1 (n-pentanol)
- Physical state: liquid
- Analytical purity: ca. 99%

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Kisslegg mouse
- Weight at study initiation: mean 29.8 g (male), 25.6 g (female)

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Details on exposure:

- Concentration of vehicle (aqueous suspension): 0.5%
- Concentration of test substance in vehicle: 2, 4, 16%
- Applied volume: 10 or 20 ml

Doses:

0.2, 0.4, 0.8, 1.6 ml/kg bw (ca. 163, 326, 652, 1304 mg/kg bw assuming a density of 0.815 g/ml)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Daily recording of signs and symptoms from the day of administration till the end of the observation period. Daily check (twice) for moribund and dead animals (once during the weekend).
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, pathology

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 326 mg/kg bw

Based on:

test mat.

Mortality:

0.2 ml/kg bw: no mortalities
0.4 ml/kg bw: 1/5 male within 24 h; 2/5 males within 7 days; 3/5 males within 14 days; 1/5 female within 7 days
0.8 ml/kg bw: 2/5 males within 24 h; 4/5 males within 7 days; 3/5 females within 48 h; 5/5 females within 7 days
1.6 ml/kg bw: 2/5 males within 1 h; 5/5 males within 7 days; 5/5 females within 1 h

Clinical signs:

0.2 ml/kg bw: directly after treatment: tachypnea, staggering and abdominal posture; after few minutes: tachypnea and staggering; from ca. 5 hours onwards: squatting posture and tachypnea; nothing abnormal was detectable in surviving animals from day 6 after treatment
0.4 ml/kg bw: directly after treatment: tachypnea, staggering and abdominal posture; after few minutes: atony, intermittent tremor and scampering gait; from ca. 5 hours onwards: squatting posture and tachypnea; nothing abnormal was detectable in surviving animals from day 13 after treatment
0.8 ml/kg bw: First day after treatment: tachypnea, staggering, atony, apathy and dyspnea. Narcosis and squatting posture partly observed; during the following days: squatting; nothing abnormal was detectable in surviving animals from day 12 after treatment
1.6 ml/kg bw: directly after treatment: tachypnea, staggering and abdominal posture; after few minutes: dyspnea and narcosis; after ca. 5 hours: dyspnea; after 2 days: intermittent tremor the 2 last animals died at day 6

Body weight:

no abnormality reported

Gross pathology:

- Survivors: nothing abnormal detected (with the exception of slightly liver size increase in the 6 animals of the 0.4 ml/kg group)
- Dead animals: loam grey staining (in 7 animals of the 1.6 ml/kg and 6 of the 0.8 ml/kg group); liver congestion hyperemia (in 3 animals of the 1.6 ml/kg and 1 of the 0.4 ml/kg group); atonic gut (in 1animal of the 0.4 ml/kg group); intra-abdominal agglutination (in 1 animal of the 0.4 mg/kg group); venous hyperemia (in 3 of the 0.8 ml/kg group); intra-intestinal diarrheal content (in 1 animal of the 0.4 ml/kg and 3 of the 0.8 ml/kg group).

Any other information on results incl. tables

Original value: LD50 = ca. 0.4 mL/kg bw