Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-063-9 | CAS number: 77-85-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline and GLP-study, validity criteria fulfilled
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- yes
- Remarks:
- 3 test substance concentrations instead of 5
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- not required
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- The test substance was pulverised and dissolved in deionised water by stirring for 18 hours before the start of the test.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: yes
- Method of cultivation:
# first, the sludge was settled and the supernatant was decanted
# after centrifuging (15 min at 4500 rpm and 20°C) the supernatant was decanted
# approx. 1 g of the wet sludge was dried to calculate the amount of wet sludge to achieve a concentration of activated sludge of 3 g/L (dry
weight) suspended solids
# the calculated amount of sludge was first dissolved in synthetic medium and than filled up to a defined end volume with deionised water
# aeration of the activated sludge at 20°C ± 2°C, daily fed with synthetic medium
- Preparation of inoculum for exposure: Before use the concentration of suspended solids of the activated sludge was determined again. 10 mL of sludge suspension were dried in order to calculate the amount of sludge suspension to achieve a concentration of activated sludge of 2 g/L (dry weight) suspended solids. The calculated amount of sludge suspension was taken and filled up to a defined end volume with deionised water. The pH was measured and adjusted to pH 6-8.
- Initial biomass concentration: 2 g/L (dry weight) suspended solids were used in order to get a test concentration of 800 mg/L suspended solids - Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Not indicated
- Hardness:
- Not indicated
- Test temperature:
- 20°C ± 2°C
- pH:
- 7.8 - 8.0 in the test solutions
- Dissolved oxygen:
- 2.6 - 7.1 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 10, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL-Erlenmeyer flasks
- Type (delete if not applicable): open
- fill volume: 250 mL
- Aeration: yes
- No. of vessels per control (replicates): 2
- No. of vessels per physical-chemical oxygen consumption control (medium with test substance and without activated sludge): 1
OTHER TEST CONDITIONS
- Adjustment of pH: yes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Oxygen content
TEST CONCENTRATIONS: 10, 100 and 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5 - dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Please refer to the table presented under "Remarks on results including tables and figures"
- Results with reference substance (positive control):
- EC50 of 3,5 - dichlorophenole is 9.2 mg/L ( 95% C.I. 3 - 24 mg/L)
- Reported statistics and error estimates:
- EC50 and the confidence interval of the reference substance was determined by probit analysis.
- Validity criteria fulfilled:
- yes
- Remarks:
- respiration rates of the 2 controls differ less than 15 % from each other; the EC50 of the reference compound 3.5-Dichlorophenol is in the range 5 - 30 mg/L
- Conclusions:
- The EC50 and the EC10 for trimethylolpropane were determined to be > 1000 mg/L.
- Executive summary:
The respiration inhibition of activated sludge from predominantly domestic sewage treatment plant by trimethylolpropane was determined according to the EU Guideline C.11 with an incubation time of 3 hours. The concentrations tested were 10, 100 and 1000 mg/L. EC50 of the reference substance 3,5 - dichlorophenole was 9.2 mg/L ( 95% C.I. 3 - 24 mg/L). The respiration rates of the 2 controls differed less than 15% from each other.
The EC50 and the EC10 for trimethylolpropane were determined to be > 1000 mg/L.
The validity criteria of the guideline are fulfilled. The deviation from the guideline used is considered to have no negative impact on the result.
Reference: Neuhahn, 2010
Reference
Test substance (nominal)
[mg/L] |
Respiration rate
[mg/L x h] |
Phys.-chem. O2consumption
[mg/L x h] |
Respiratory rate - phys.-chem. O2consumption [mg/L x h] |
Inhibition
[%] |
10 |
32.0 |
0.0 |
32.0 |
0.0 |
100 |
34.0 |
0.0 |
34.0 |
0.0 |
1000 |
32.0 |
0.0* |
32.0 |
0.0 |
Control mean |
30.8 |
- |
||
Control 1 |
30.0 |
- |
||
Control 2 |
31.5 |
- |
*The physico-chemical oxygen consumption has been determined at 1000 mg/L test item concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Assessment is by read-across to the source substance trimethylolpropane (CAS No: 77-99-6).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
Tato webová stránka používá cookies, aby se vám naše stránky používaly co nejlépe.