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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
19. Aug 1969 - 27. Aug 1969
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment. However, there is no analytical verification of the atmosphere concentrations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1970
Report date:
1970

Materials and methods

Principles of method if other than guideline:
Exposure of rats to an atmosphere saturated with vapours of isopropoxypropylamine generated at the temperature of 20 °C.
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Reference substance name:
Isopropoxypropylamine
IUPAC Name:
Isopropoxypropylamine
Constituent 2
Chemical structure
Reference substance name:
3-isopropoxypropylamine
EC Number:
220-816-2
EC Name:
3-isopropoxypropylamine
Cas Number:
2906-12-9
Molecular formula:
C6H15NO
IUPAC Name:
3-isopropoxypropylamine
Details on test material:
- Name of test material (as cited in study report): Isopropoxy-propylamin
- Analytical purity: > 99 %

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 163 g

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
other: unchanged (no vehicle)
Analytical verification of test atmosphere concentrations:
no
Remarks on duration:
1 h, 3 h or 8 h
Concentrations:
1 h exposure: 48.5 mg/L
3 h exposure: 19.25 mg/L
8 h exposure: 18.5 mg/L
(nominal concentrations not verified by analytics).
No. of animals per sex per dose:
1 h exposure: 6
3 h and 8 h: 3
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Frequency of weighing: day 0 and day 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC0
Effect level:
other: > saturated vapor atmosphere at 20°C
Exp. duration:
1 h
Remarks on result:
other: No mortality
Sex:
male/female
Dose descriptor:
LC50
Effect level:
other: > saturated vapor atmosphere at 20°C
Exp. duration:
3 h
Remarks on result:
other: mortality 1/6
Sex:
male/female
Dose descriptor:
LC50
Effect level:
other: < saturated vapor atmosphere at 20°C
Exp. duration:
8 h
Remarks on result:
other: mortality 5/6
Mortality:
3 h exposure: 1 animal died within 2 h of exposure.
8 h exposure: 3 animals died within 4.5 h of exposure and 2 further animals died within the end of exposure.
Clinical signs:
other: Initially escape attempts, irritation of the mucous membranes, dyspnoea. After the 3 and 8 h exposure also convulsions, staggering, corrosion of eyes and noses. 48 h post exposure in the 8 h exposure group intermittent respiration, respiratory sounds and
Body weight:
All surviving animals gained weight.
Gross pathology:
8 h exposure: 5x bloody nose crusts, lungs filled with blood, corroded snouts, sodden fur.

Any other information on results incl. tables

Mortality:

   24 h  7 days  14 days    
 1 h exposure 0 /12  0/12  -    
 3 h exposure  1/6  1/6  -    
 8 h exposure  5/6  5/6  5/6    

Weight (g):

   day 0  day 7  day 14    
 1 h exposure  156  177 -    
 3 h exposure 176  227    
 8 h exposure 162   - 200      

The inhalation of a highly enriched/saturated vapor-air-mixture may represent a hazard (mortality within 3 h). There is indication that the test substance causes local irritation to exposed tissues (including respiratory tract).

Applicant's summary and conclusion

Executive summary:

In a test to demonstrate the toxicity of an atmosphere saturated with vapours of the volatile components of isopropoxypropylamine at the temperature chosen for vapour generation (20 °C) (BASF, 1970), 3 rats per sex were exposed sequentially to the vapours, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 1 h, 3 h or 8 h. The documentation of clinical signs was performed over a period of 7 days. During the 3 h exposure, 1/6 animal died within 2 h of exposure and for the 8 h exposure, 3/6 animals died within 4.5 h of exposure and 2/6 further animals died within the end of exposure. Escape attempts, irritation of the mucous membranes and dyspnoea were observed initially. After the 3 and 8 h exposure, convulsions, staggering, corrosion of eyes and noses were also noted. 48 h post exposure in the 8 h exposure group intermittent respiration, respiratory sounds and ruffled fur were noted. All surviving animals gained weight. For the 8 h exposure, bloody nose crusts, lungs filled with blood, corroded snouts, and sodden fur were observed in 5 rats.

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