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Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study following national standard; result relates to nominal concentration, test item concentration not verified
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h
Test temperature:
20°C
pH:
6.3 - 10.4
Nominal and measured concentrations:
- Nominal test concentrations: 15.63, 31.25, 62.5, 125, 250, 500, 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glas penicillium vessel, closed
- Test volume: 10 mL
- Aeration: none
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 mL of a Pseudomonas suspension adjusted to a turbidity of 10 TE/F

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium according to DIN 38412, part 8 (draft)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Photometric determination of light extinction after 17 hours of exposure
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
83.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: light extinction
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
182 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: light extinction
Duration:
17 h
Dose descriptor:
other: EC90
Effect conc.:
374.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: light extinction

 Conc. (nominal) [mg/L]  Extinction  Difference to Control [%]
 Control 0.287 100 
 15.63 0.339  117.93
 31.25  0.314  109.14
 62.5  0.279  97.04
 125  0.218     75.89
 250  0.055  19.15
 500  0.002  0.78
1000  0.001  0.44

                                  pH values

 Conc. (nominal) [mg/L]  pH after 0 h  pH after 17 h
 Control 7.1 6.5 
 15.63 7.3  6.3
 31.25  7.5  6.3
 62.5  8.3  6.4
 125  9.5     6.6
 250  9.9  7.6
 500  10.2  8.6
1000  10.4  9.1

Description of key information

A test on the inhibitory action of 3 -isopropoxypropanamine on the growth of Pseudomonas putida gave a 17h-EC10 of 83.3 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
83.3 mg/L

Additional information

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