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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
None.
Vehicle:
no
Details on test solutions:
Test solutions were standard solutions as described in the OECD 202 guideline.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Clone 5 or Clone A
- Source: Laboratory bred on Elendt M7
- Feeding during test: none
- Food type during parent breeding: Raphidocelis subcapitata
- Age: less than 24-h old
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
No data.
Test temperature:
19-21°C (min - max)
pH:
Comprised between 7.9 and 8.7 from 0 to 47 mg/L test concentrations. Comprised between 9 and 9.1 at 75 mg/L. Comprised between 9.4 and 9.7 at 120 mg/L.
Dissolved oxygen:
7.5-8.0 (min-max)
Salinity:
Freshwater
Conductivity:
No data
Nominal and measured concentrations:
Stability of the test item was checked in a previous study on daphnia in the same laboratory. It was demonstrated that the test item is stable. Therefore, nominal concentrations were used and not monitored in the present study: control ; 7.2 ; 11.4 ; 18.4 ; 29.3 ; 46.9 ; 75 and 120 mg/L.
Details on test conditions:
TEST SYSTEM
- Type: tightly closed with PTFE septa to avoid evaporation
- Material, size, headspace, fill volume: 120 mL, no headspace
- Aeration: None
- Renewal rate of test solution (frequency/flow rate): no renewal during the test (static test)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: none

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 24 and 48 hours.

RANGE-FINDING STUDY
- Test concentrations: 37 - 200 mg/L
- Results used to determine the conditions for the definitive study: EC50 < 37 mg/L
Reference substance (positive control):
not required
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
65 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Details on results:
After 48 hours exposure, immobilisation reached 65% at 75 mg/L and 100% at 120 mg/L. It remained between 0 and 5% (i.e. one daphnid) in the other treatments. As a result, the 48h-EC50 of the test item is considered 65 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
48h-EC50 of 3-isopropoxypropanamine is 65 mg/L.
Executive summary:

An OECD 202 study was carried out in order to assess the acute toxicity of 3 -isopropoxypropanamine (IPOPA) to daphnia. Tested concentrations were 0 ; 7.2 ; 11.4 ; 18.4 ; 29.3 ; 46.9 ; 75 and 120 mg/L. Concentrations were not monitored analytically, but stability of the test item was checked in the range-finder. It was demonstrated that the test item is stable. Therefore, nominal concentrations were used and no analytical monitored was deemed necessary. The highest concentration had a pH above 9, which could explain the 100% mortality observed. In the 75 mg/L group, the pH was at the limit of the acceptable for this kind of test (9.0 -9.1). 65% mortality was observed. In the absence of any toxicity testing with neutralized substance, it is difficult to ascertain whether this mortality is due to pH or the substance itself. Giving that the range finder gave an EC50 < 37 mg/L, it is considered that the 65% immobilisation observed at 75 mg/L is not pH related. Therefore, an EC50 can be derived and is equal to 65 mg/L.

Description of key information

An OECD 202 study was carried out in order to assess the acute toxicity of 3 -isopropoxypropanamine (IPOPA) to daphnia. Tested concentrations were 0 ; 7.2 ; 11.4 ; 18.4 ; 29.3 ; 46.9 ; 75 and 120 mg/L. Concentrations were not monitored analytically, but stability of the test item was checked in the range-finder. It was demonstrated that the test item is stable. Therefore, nominal concentrations were used and no analytical monitored was deemed necessary. The highest concentration had a pH above 9, which could explain the 100% mortality observed. In the 75 mg/L group, the pH was at the limit of the acceptable for this kind of test (9.0 -9.1). 65% mortality was observed. In the absence of any toxicity testing with neutralized substance, it is difficult to ascertain whether this mortality is due to pH or the substance itself. Giving that the range finder gave an EC50 < 37 mg/L, it is considered that the 65% immobilisation observed at 75 mg/L is not pH related. Therefore, an EC50 can be derived and is equal to 65 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
65 mg/L

Additional information