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Toxicity to aquatic algae and cyanobacteria

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Description of key information

An algal growth study was conducted on the test material, this study was conducted according to OECD guideline 201 and EU Method C3. The results of this study showed Sodium Glucoheptonate to have an EC50 value of 790 mg/L and a NOEC of 100 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
790 mg/L
EC10 or NOEC for freshwater algae:
100 mg/L

Additional information

Introduction:

A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) No 761/2009 and the US EPA Draft Ecological Effects Test Guideline OPPTS 850.5400.

 

Methods:

Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to an aqueous solution of the test item at concentrations of 10, 32, 100, 320 and 1000 mg active ingredient (ai)/L (three replicate flasks per concentration) for 96 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C.

 

Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter®Multisizer Particle Counter.

Results:

Exposure of Pseudokirchneriella subcapitata to the test item gave the following results:

 

Time Point

(Hours)

Response Variable

EC50(mg ai/L)

95% Confidence Limits (mg ai/L)

No Observed Effect Concentration (NOEC) (mg ai/L)

Lowest Observed Effect Concentration (LOEC) (mg ai/L)

72

Growth Rate

510

 

*

 

32

100

Yield

150

120

-

190

32

100

Biomass

160

140

-

190

32

100

96

Growth Rate

790

 

*

 

100

320

Yield

220

180

-

260

10

32

Biomass

180

140

-

210

32

100


Analysis of the test preparations at 0 and 96 hours showed measured test concentrations to range from 93% to 105% of nominal and therefore the results are based on nominal test concentrations only, as this coincides within the acceptable range of between 80 and 120%.

A re-growth test was performed which showed the test item to be algistatic in effect.