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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Description of key information

The study was conducted according to GLP and according to OECD guideline 202.  The test item was exposed to Daphnia magna for 48 hours under static test conditions. The EC50 value after 48 hours was 1000 mg ai/L and the NOEC was 1000 mg/ai/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1 000 mg/L

Additional information

Introduction:

A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the US EPA Draft Ecological Effects Test Guidelines OPPTS 850.1010 (April 1996) and the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.

 

Methods:

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at concentrations of 100, 180, 320, 560 and 1000 mg active ingredient (ai)/L for 48 hours at a temperature of 20°C to 21°C under static test conditions. The number of immobilized Daphnia were recorded after 24 and 48 hours.

 

Results:

The 48-Hour EC50 for the test item to Daphnia magna based on nominal test concentrations was greater than 1000 mg ai/L. The No Observed Effect Concentration was 1000 mg ai/L.

 

Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 80% to 83% of nominal value and so the results are based on nominal test concentrations only.