Carbonic acid, 4-nitrophenyl 5-thiazolylmethyl ester, hydrochloride (1:1)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 May - 30 June, 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under GLP and OECD method

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Directive 92/69/EEC, Method B3 Part 3

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

414-490-2

EC Name:

-

Cas Number:

154212-59-6

Molecular formula:

C11H9ClN2O5S

IUPAC Name:

4-nitrophenyl (1,3-thiazol-5-yl)methyl carbonate hydrochloride

Test animals

Species:

rat

Strain:

other: Crl;CD(SD)BR

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles Rivers, MI for males, Raleigh, NC for females.
- Age at study initiation: eight to ten weeks
- Weight at study initiation: 227 - 291 g
- Fasting period before study: n/a
- Housing: ventilated stainless steel hanging cages.
- Diet: Certified Rodent Chow ad libitum
- Water : ad libitum
- Acclimation period: Eight days

ENVIRONMENTAL CONDITIONS
- Temperature (°F): 70 -72°
- Humidity (%): ambient
- Photoperiod (hrs dark / hrs light): 12 hrs light/12hrs dark

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

water

Details on dermal exposure:

TEST SITE
- Area of exposure: 50 x 50mm

REMOVAL OF TEST SUBSTANCE
- Washing (if done):
- Time after start of exposure:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit):
- Concentration (if solution):

VEHICLE
- Amount(s) applied (volume or weight with unit):
- Concentration (if solution):

Duration of exposure:

24 hours

Doses:

2g/kgBW

No. of animals per sex per dose:

5 males/5 females

Details on study design:

Fill in wht you can==

- Duration of observation period following administration: 14 days
- Frequency of observations: Daily
- Frequency of weighing: Weekly
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

No rats died during study

Clinical signs:

other: except for pale eyes in a single female rat on day 3 of observation, there was no irritation at the treatment site

Gross pathology:

No apparent treatment related effects on gross pathology were observed during 2 week observation period.

Other findings:

Very slight to slight erythema and multiple small scabsat the site of exposure were noted in nearly all male and female rats. Very slight to slight edema at the exposure site were noted in three females and scaling of the skin in two females.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute lethal dermal dose (LD50) to rats was demonstrated to be greater than the limit dose of 2mg/kg bodyweight