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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
10 Sept -13 Sept, 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Done under GLP and OECD methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Version / remarks:
Method C3, Directive 92/69/EEC
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes
Remarks:
87/18 EEC

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
414-490-2
EC Name:
-
Cas Number:
154212-59-6
Molecular formula:
C11H9ClN2O5S
IUPAC Name:
4-nitrophenyl 1,3-thiazol-5-ylmethylcarbonate hydrochloride

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Test substance dissolved in 1% Tween 80 dimethylformamide
Nominal test concentrations: 0.22, 0.46, 1.0, 2.2, 4.6 and 10mg/l
Mean measured test concentrations: 0.039, 0.048, 0.075, 0.12, 0.18 and 0.27mg/l

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: CCAP 276/20
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa c/o Freshwater Biological Association, Cumbria, UK.

Study design

Test type:
semi-static
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
20.4 - 24.12°C
Nominal and measured concentrations:
Nominal test concentrations: 0.22, 0.46, 1.0, 2.2, 4.6 and 10mg/l
Mean measured test concentrations: 0.039, 0.048, 0.075, 0.12, 0.18 and 0.27mg/l
Details on test conditions:
100ml of test or control culture incubated for 72hrs under continuous illumination of approx. 7,000 lux with orbital shaker at 120 cycles/min.
No media renewal during test.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.19 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 0.16 - 0.21
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.27 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.12 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.075 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Details on results:
Starting from the real concentrations of exposure:
ECr50(0-72h) : >0.27mg/L measured
No observed effect level: 0.075mg/L measured (area under curve)
0.12mg/L measured ( 0- 72 hr growth rate)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 0-72hr ErC50 of test substance was calculated to be >0.27 mg/l

The 72hr EbC50 of test substance was calculated to be 0.19mg/l

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