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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 October - 15 November, 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1997

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: OECD Guideline 202 Part 2 (Testing of Chemicals)
Qualifier:
according to guideline
Guideline:
EPA OPP 72-4 (Fish Early Life-Stage and Aquatic Invertebrate Life-Cycle Studies)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
414-490-2
EC Name:
-
Cas Number:
154212-59-6
Molecular formula:
C11H9ClN2O5S
IUPAC Name:
4-nitrophenyl 1,3-thiazol-5-ylmethylcarbonate hydrochloride

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations (measured): 0.036, 0.066, 0.125, 0.270, 0.415mg/l plus Control plus Solvent Control.
- Sampling method: Duplicate aqueous samples (40 to 50ml) of solvent control and each treatment solution were analyzed immediately after preparation on days 0, 3, 7, 14, 17 and 19
- Sample storage conditions before analysis: 20±2°C

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name:
- Strain/clone: Straus
- Source: Institut National de Recherche Chimique Applique (IRChA) France
Feeding during test
- Food type: green alga Chlorella vulgaris strain CCAP211/12
- Frequency: 0.5 - 1.0ml at least 5 times weekly

Study design

Test type:
other: treatment solutions renewed 3 times per week.
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
20±2°C
pH:
7.9 - 8.0
Dissolved oxygen:
8.4 - 8.7mgO2/L
Nominal and measured concentrations:
Measured concentrations of test substance were between 4 - 36 % of the nominal values.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: 1 litre glass beakers with loosely fitted foam bung.
- Material, size, headspace, fill volume: 500ml Elendt M4 culture medium
- Aeration:
- Renewal rate of test solution (frequency/flow rate): 3/wk on days 3, 5, 7, 10, 12, 14, 17 and 19.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4


TEST MEDIUM / WATER PARAMETERS
Diluent water control and solvent control treatmentswere prepared. Solvent controls contained 100µl DMF:Tween 80 as auxillary solvent.

OTHER TEST CONDITIONS
- Photoperiod: 16hrs light, 8hrs dark
Reference substance (positive control):
yes
Remarks:
100µl DMF:Tween 80

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
0.45 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: parental mortality
Remarks on result:
other: 95%CL: 0.35 -0.57mg/L
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
0.33 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: 95%CL:0.30 - 0.37mg/l
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
0.34 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: 95%CL: 0.32 - 0.37mg/l
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.125 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
Values based on mean measured concentrations calculated .
Limit of detection: 0.02mg/l
Reported statistics and error estimates:
Mean measured concentrations of A-87439.1 in test solutions on each sampling occassion were variable and ranged from 54.8% to 126% of nominal for freshly prepared solutions.
Analysis of solvent stock solutions of A-87439.1 showed the mean measured concentration to be 95.3% of nominal.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Prolonged exposure of Daphnia magna to A-87439.1 caused inhibition of reproduction with EC 50 0.34 mg/l and NOEC of 0.125 mg/l measured concentrations. LOEC was 0.270 mg/L.

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