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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 March 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: done under GLP and OECD method

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: EC Directive 87/302 'Activated Sludge, Respiration Inhibition test'
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
414-490-2
EC Name:
-
Cas Number:
154212-59-6
Molecular formula:
C11H9ClN2O5S
IUPAC Name:
4-nitrophenyl 1,3-thiazol-5-ylmethylcarbonate hydrochloride

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Test substance concentration: 22 mg/L (nominal)
Reference substance concentration: 3.2, 10 and 32 mg/ml

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Activated sewage sludge maintained at 20±2°C, continuously aerated and used within 24hrs.

Study design

Test type:
other: continuously aerated
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Hardness:
approx 120 -180mg/L (as CaCO3)
Test temperature:
20°C
pH:
8.1
Dissolved oxygen:
6.5 - 2.5mgO2/L
Details on test conditions:
TEST SYSTEM: series
- Test vessel: 1 litre glass flask
- Aeration: yes
- No. of vessels per concentration (replicates): not stated
- No. of vessels per control (replicates): not stated
- No. of vessels per vehicle control (replicates): Not stated

test has test substance+synthetic sewage + inoculum
controls has synthetic sewage + inoculumn only

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated and Reverse Osmosis

TEST CONCENTRATIONS
Test substance concentration: 22 mg/L
Reference substance concentration: 3.2, 10 and 32 mg/ml
Reference substance (positive control):
yes
Remarks:
3.5 dichlorophenyl

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 22 mg/L
Nominal / measured:
nominal
Details on results:
22mg/l was the highest concentration that could be prepared due to the limited solubility of the test substance in water.
Results with reference substance (positive control):
EC50 (3 hours): 10mg/l
Reported statistics and error estimates:
Variation in respiration rates of Controls 1 and 2: ±3%
Variation in respiration rates of Solvent Controls 1 and 2: ±8%

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
(i) Respiration rate for the 2 control cultures: within 15% of each other at 3 hrs contact time. (ii) EC50 of 3,5-dichlorophenol at 3 hours contact time: 5 - 30mg/L
Conclusions:
The EC50 (respiration inhibition) values for A-87439.1 with activated sewage sludge has been determined as >22mg/L (nominal concentration) for the 3 hour contact time.

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