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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Link to relevant study record(s)

Description of key information

Key value for chemical safety assessment

Additional information

Based on the considerations described in the document "Read-across substantiation C5-6 branched alkylmethyl-ethers” (incl. the supporting references Tuppurainen et al., 2007 and Niska et al., 2008), it can be concluded that the available information of TAME can be used to predict the basic toxicokinetics of the substance ‘C5-6 branched alkylmethyl-ethers’ with sufficient certainty.



Based on the available data, TAME is absorbed efficiently from the rat intestine. Hepatic first-pass metabolism was not observed. TAME is rapidly absorbed from lungs; in a study with human volunteers, the respiration uptake was approximately 50%. The maximum plasma concentration of humans after 50 ppm TAME exposure for 4 hours was about 13 μmol/L.


Distribution and elimination

In rats, TAME is distributed evenly throughout the body and is eliminated via respiration, urine and faeces, urine being the main route of elimination while excretion in faeces is only a few percent at maximum. The elimination from human blood was rapid after the end of inhalation exposure and it occurred in two phases. The blood half-lifes were between 1.2 and 6.3 hours with relatively big individual differences between test subjects. The limit of TAME detection was reached after 12 hours. In rat, the removal via respiration increases with higher doses.

The kinetics of excretion of TAME were identical after ingestion and inhalation exposure.



In humans, TAME is metabolised to tert-amyl alcohol (TAA) primarily by cytochrome 2A6 mainly present in liver. The first metabolic step releases tert-amyl alcohol and formaldehyde. The main human urine metabolites are 2-methyl-2,3-butanediol, 2-hydroxy-2-methylbutyrate and 3-hydroxy-3-methylbutyrate. Free and conjugated TAA and TAME were only minor metabolites in urine. In rats, 2-methyl-2,3-butanediol and its glucuronide were major TAME metabolites recovered in urine.

The metabolic pathway of TAME is identical after ingestion and inhalation exposure.


Information used for DNEL derivation

The inhalation and dermal absorption percentages used for DNEL derivation (in case of applying route-to-route extrapolation) are 50% and 0.4%, respectively.