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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1973
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1973
Report date:
1973

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
No exact guideline or method detailed in report.
GLP compliance:
not specified
Remarks:
pre-GLP report
Test type:
fixed dose procedure
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
(E)-2-methyl-3-phenylprop-2-enal
Cas Number:
15174-47-7
IUPAC Name:
(E)-2-methyl-3-phenylprop-2-enal
Test material form:
liquid
Specific details on test material used for the study:
Test material was described with the generic name "alpha-methylcinnamaldehyde". As manufactured by Innospec, the product met the criteria of > 80% trans-isomer, supporting that the test material was the isomer better described as CAS 15174-47-7.

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
Compound No. RIFM 72-257

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
not specified
Doses:
1.64, 2.05, 2.56, 3.20, 5.00 g/Kg
No. of animals per sex per dose:
10 animals per dose, no sex details given
Control animals:
not specified

Results and discussion

Effect levels
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
ca. 2 050 mg/kg bw
95% CL:
> 1.83 - < 2.27
Mortality:
On day 1 post oral exposure mortality was observed as follows:
1.64 g/Kg - 2 rats dead
2.05 g/Kg - 4 rats dead
2.56 g/Kg - 8 rats dead
3.20 g/Kg - 10 rats dead
5.00 g/Kg - 10 rats dead
Clinical signs:
other: The animals which died experienced sluggishness prior to death

Any other information on results incl. tables

Table 1. Distribution of Mortality

Dose

(g/Kg)

Observation Day

Mortality

1

2

3

4

5

6

7

8

9

10

11

12

13

14

1.64

2

0

0

0

0

0

0

0

0

0

0

0

0

0

2/10

2.05

4

0

0

0

0

0

0

0

0

0

0

0

0

0

4/10

2.56

8

0

0

0

0

0

0

0

0

0

0

0

0

0

8/10

3.20

10

0

0

0

0

0

0

0

0

0

0

0

0

0

10/10

5.00

10

0

0

0

0

0

0

0

0

0

0

0

0

0

10/10

Applicant's summary and conclusion

Interpretation of results:
other: Not classified
Remarks:
Regulation (EC) No 1272/2008 on classification, labelling and packaging (CLP) of substances and mixtures
Conclusions:
Based on observed mortality, the acute oral LD50 of Alpha methyl cinnamic aldehyde was determined to be 2050 mg/Kg (95% confidence interval = 1830 – 2270 mg/Kg).
Executive summary:

In a key fixed dose oral toxicity study, the test material (Alpha methyl cinnamic aldehyde) was orally administered to rats (10/dose) at doses of 1.64, 2.05, 2.56. 3.20, or 5.0 g/Kg.

 

All animals treated at ≥3.20 g/Kg died on day one. At 1.64 g/Kg, 2/10 animals died while 4/10 and 8/10 animals died post exposure to the test material at doses of 2.05 and 2.56 g/Kg, respectively.

 

The acute oral LD50 of Alpha methyl cinnamic aldehyde was therefore determined to be 2050 mg/Kg (95% confidence interval = 1830 – 2270 mg/Kg).